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| Chromosome and Gene Mapping Homology between River Buffalo, Cattle and Sheep using Molecular Markers |
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Othman E. Othman |
Abstract:
Chromosomal localization of sixteen bovine microsatellites in river buffalo
has been assigned in this study, using polymerase chain reaction and buffalo-hamster
somatic cell hybrids. These tested microsatellites were previously assigned
to sheep chromosomes. This study also aimed to confirm that the chromosome band
identity between river buffalo, cattle and sheep is a good indicator of genetic
homology between these closely related species. The correlation coefficients
between these tested microsatellites and other markers- representing syntenic
groups and chromosomes in river buffalo- was calculated to assign these microsatellites
to river buffalo chromosomes (BBU)). The results showed that BM719 is assigned
to BBU5q, BM827 to BBU12, BM1818 to BBU2p, BM1824 to BBU1q, BM2113 to BBU2q,
CSSM015 to BBU3p, CSSM034 to BBU4q, CSSM037 to BBU3q, CSSM043 to BBU1p, CSSM058
to BBU21, ILSTS011 to BBU15, ILSTS013 to BBU10, ILSTS019 to BBU5p, ILSTS029
to BBU6, ILSTS054 to BBU20 and ILSTS060 to BBU4p. The result also showed that
16 tested microsatellites are localized in river buffalo, cattle and sheep on
the equivalent chromosomes, which have chromosome- band homology, in these closely
related species belonging to Bovidae family, which has a high degree of chromosome
conservation between its members and where the bi-armed autosomes are formed
by centric fusions of acrocentric autosomes.
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References
Citation
Report Citation
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How to cite this article:
Othman E. Othman , 2004. Chromosome and Gene Mapping Homology between River Buffalo, Cattle and Sheep using Molecular Markers. Biotechnology, 3: 119-125.
DOI: 10.3923/biotech.2004.119.125
URL: https://scialert.net/abstract/?doi=biotech.2004.119.125
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