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Biotechnology
  Year: 2004 | Volume: 3 | Issue: 2 | Page No.: 114-118
DOI: 10.3923/biotech.2004.114.118
 
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Somatic Embryogenesis in Phoenix dactylifera L: Effect of Exogenous Supply of Sucrose on Proteins, Sugars, Phenolics and Peroxidases Activities During the Embryogenic Cell Suspension Culture

J. Zouine and I. El Hadrami

Abstract:
The present study was conducted to determine the effect of three concentrations of usually used (1.5, 3 and 6%) of sucrose on the proliferation of date palm cell suspension culture. The medium containing 3% sucrose, leads to best results than 1.5 and 6% sucrose especially for JHL cultivar. In fact, when sucrose concentration increased from 1.5 to 3%, the embryogenic mass significantly increased from 2.15 to 14.6 g per flask and 8.7 to 10 g per flask, respectively for JHL and BSTN cultivars. The biochemical analysis showed that the maturation medium with 3% sucrose significantly increased the proteins (14.9 mg g-1 FW for JHL cultivar and 5 mg g-1 FW for BSTN cultivar) and sugars contents (22.5 and 22.7 mg g-1 FW, respectively, for JHL and BSTN cultivars) in the embryogenic mass. In addition, as compared to 1.5 and 6%, the use of 3% sucrose enhanced phenolics contents (0.69 mg g-1FW) and peroxidases activities (2.9.103 n Kat g-1FW) in BSTN cultivar.
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How to cite this article:

J. Zouine and I. El Hadrami , 2004. Somatic Embryogenesis in Phoenix dactylifera L: Effect of Exogenous Supply of Sucrose on Proteins, Sugars, Phenolics and Peroxidases Activities During the Embryogenic Cell Suspension Culture. Biotechnology, 3: 114-118.

DOI: 10.3923/biotech.2004.114.118

URL: https://scialert.net/abstract/?doi=biotech.2004.114.118

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