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Biotechnology
  Year: 2003 | Volume: 2 | Issue: 1 | Page No.: 1-7
DOI: 10.3923/biotech.2003.1.7
 
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Optimization of Various Parameters for the Production of Glucose Oxidase from Rice Polishing Using Aspergillus niger

Hafiz M. Hamid, Khalil-ur-Rehman , M. Anjum Zia and M. Asgher

Abstract:
The enzyme glucose oxidase was produced by fermentation technology, using Aspergillus niger as fermentation organism and rice polishing as substrate. Maximum production of enzyme (3.42 U mL-1) was recovered at substrate level of 2% after 36 h of submerged fermentation. The pH for the optimal production of enzyme was found to be 4. Addition of salts such as urea (0.3%), CaCO3 (0.04%) and KH2PO4 (0.6%) into the fermentation medium enhanced enzyme production while MGSO4.7H2O was found to inhabit microbial growth and glucose oxidase production by A. niger.
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How to cite this article:

Hafiz M. Hamid, Khalil-ur-Rehman , M. Anjum Zia and M. Asgher , 2003. Optimization of Various Parameters for the Production of Glucose Oxidase from Rice Polishing Using Aspergillus niger. Biotechnology, 2: 1-7.

DOI: 10.3923/biotech.2003.1.7

URL: https://scialert.net/abstract/?doi=biotech.2003.1.7

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