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Asian Journal of Scientific Research
  Year: 2008 | Volume: 1 | Issue: 5 | Page No.: 525-531
DOI: 10.3923/ajsr.2008.525.531
 
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Monoclonal Antibody Production: Media Optimization for Enhancement the Cell Viability of Hybridoma Cell

Maizirwan Mel, Maizura Mat Saad, Yumi Zuhanis Hasyun Hashim and Mohamad Ramlan Mohamed Salleh

Abstract:
Media optimization of RC1 hybridoma cell culture for monoclonal antibody production was carried out in T-Flask experiment. The three identified important variables to affect the cell viability were studied. By using Central Composite Design of Response Surface Methodology (STATISTICA v 6.1) has shown that cell viability was mainly affected by glutamine, serum and NaCO3 concentration, respectively. Among the 16 runs tested, Run 11 indicated the best viability of the cell (>80% for five days). The critical values were obtained at 13.5, 1.68 and 0.87% for serum, glutamine and NaCO3, respectively. These data were very significant where the p-values obtained for glutamine, serum and NaCO3 were 0.000069, 0.003968 and 0.342151 (R2 = 0.95476 and R Adj. = 0.88691), respectively.
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How to cite this article:

Maizirwan Mel, Maizura Mat Saad, Yumi Zuhanis Hasyun Hashim and Mohamad Ramlan Mohamed Salleh, 2008. Monoclonal Antibody Production: Media Optimization for Enhancement the Cell Viability of Hybridoma Cell. Asian Journal of Scientific Research, 1: 525-531.

DOI: 10.3923/ajsr.2008.525.531

URL: https://scialert.net/abstract/?doi=ajsr.2008.525.531

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