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Asian Journal of Scientific Research

Year: 2008 | Volume: 1 | Issue: 3 | Page No.: 288-292
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Authors

K.J. Lee

K.H. Row

In-Chul , Jun

Keywords

Research Article

Preparative Separation of Isoflavones from Korean Soybean by HPLC

K.J. Lee, K.H. Row and In-Chul , Jun
In this study, analytical and preparative HPLC systems were utilized to obtain isoflavones from Korean soybean. The mobile phases were the ternary system of water/acetonitrile/acetic acid. The optimum operating conditions were experimentally determined with the analytical column (C18, 0.46x25 cm 5 micron and the preparative column packed with 15 micron. The experimental variables were the gradient conditions and mobile phase composition. The aglycones isoflavones of daidzein, glycitein and genistein were isolated and identified. The yield of solid extract was 5%, while the weight percentage of genistin, daidzein and genistein was 0.2 wt.%. It was found that the total amount of the aglycone isoflavones of daidzein and genistein extracted from the Korean soybean was 23.18 μg g-1.
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