Abstract:
Serodiagnosis of Haemonchus contortus by ELISA using adult crude
somatic and excretory-secretory antigens of Haemonchus contortus
was tested in the present study. The diagnostic sensitivity of ELISA using
excretory secretory antigens was 87.5% which was significantly higher
compared to crude somatic antigen 72.22%. Mean ELISA absorbance value
of excretory-secretory antigens was significantly higher corresponding
to crude somatic antigens. Excretory secretory antigens showed 92.02%
specificity compared to 76.81% of crude somatic antigens (p<0.05).
The results revealed that excretory secretory antigens are very sensitive
and may be useful as a supplementary method for diagnosis of Haemonchosis
in ruminants.
R.A. Mir, M.Z. Chishti, M.A. Zargar, Hidayatullah Tak and S.A. Ganie, 2008. Excretory-Secretory Antigens are Better than Crude Antigens for
Serodiagnosis of Haemonchus contortus. Asian Journal of Scientific Research, 1: 171-175.