Effect of 6-Benzyladenine Purine, α-Naphthalene Acetic Acid, Coconut Water and Potato Extract on Micropropagation of Ensete glaucum from Vietnam

Research Article

**Effect of 6-Benzyladenine Purine, α-Naphthalene Acetic Acid, Coconut Water and Potato Extract on Micropropagation of Ensete glaucum from Vietnam**

Xuan Duong Vu, Thi Tam Tien Ha, Thanh Loan Pham, Luong Thi Hien Dao and Phi Bang Cao

Background and Objective: Ensete glaucum (Roxb.) Cheesman is widely dispersed and has ornamental value as well as potential medicinal benefits. This research aimed to discover the effect of BAP, NAA and two organic additives, coconut water and potato extract on shooting and rooting during in vitro propagation of Ensete glaucum collected from Xuan Son Nation Park, Phu Tho Province, Vietnam. Materials and Methods: For shoot production, sterilized shoot tip explants of E. glaucum (1 cm in height) were placed in cultured media containing BAP at different concentrations (0, 1.0, 2.0, 3.0 and 4.0 mg L^–1) combined with 0.2 mg L^–1 NAA for the first experiment. Coconut water (CW) (0, 50, 100, 150 and 200 mL L^–1) and potato extract (PE) (0, 10, 20, 30 and 40 g L^–1) combined with BAP at the optimum concentration obtained from the previous experiment was used for the second experiment. For rooting, explants of E. glaucum (3 cm in height) were transferred in cultured media containing different concentrations of NAA (0, 0.5, 1.0, 1.5 and 2.0 mg L^–1) combined with 0.2 mg L^–1 BAP for the first experiment. In the second experiment, explants of E. glaucum were cultured in the medium added CW (0, 50, 100, 150 and 200 mL L^–1) and PE (0, 10, 20, 30 and 40 g L^–1) combined with NAA at the optimum concentration obtained from the previous experiment to investigate the effect of these organic additives. Obtained data were statistically analyzed by using Duncan’s Multiple Range Test (p = 0.05). Results: The BAP at the concentration of 3 mg L^–1 combined with 0.2 mg L^–1 NAA gave the highest mean number of shoots produced per explant and shoot length while, 2 mg L^–1 BAP combined with 0.2 mg L^–1 NAA acquired the highest mean number of leaves. The NAA at 0.5 mg L^–1 (combined with BAP at 0.2 mg L^–1) was the most favorable for the rooting of E. glaucum. Medium supplemented with CW (100 mL L^–1) or PE (30 g L^–1 for shooting and 20 g L^–1 for rooting, respectively) resulted in an increase in shoot multiplication and root generation during micropropagation of E. glaucum. Conclusion: The present study first represents an investigation of the effect of BAP, NAA, coconut water and potato extract on micropropagation of E. glaucum collected in Vietnam.

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Xuan Duong Vu, Thi Tam Tien Ha, Thanh Loan Pham, Luong Thi Hien Dao and Phi Bang Cao, 2023. Effect of 6-Benzyladenine Purine, α-Naphthalene Acetic Acid, Coconut Water and Potato Extract on Micropropagation of Ensete glaucum from Vietnam. Asian Journal of Plant Sciences, 22: 374-381.

DOI: 10.3923/ajps.2023.374.381

URL: https://scialert.net/abstract/?doi=ajps.2023.374.381

Asian Journal of Plant Sciences

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Xuan Duong Vu

Thi Tam Tien Ha

Thanh Loan Pham

Luong Thi Hien Dao

Phi Bang Cao

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Research Article

**Effect of 6-Benzyladenine Purine, α-Naphthalene Acetic Acid, Coconut Water and Potato Extract on Micropropagation of Ensete glaucum from Vietnam**

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