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Asian Journal of Plant Sciences
  Year: 2019 | Volume: 18 | Issue: 3 | Page No.: 131-138
DOI: 10.3923/ajps.2019.131.138
 
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Mass Propagation of Urena lobata L. Hairy Root Possessing α-Glucosidase Inhibitory Activity by Using Suitable Culture Conditions

Vu Thi Bach Phuong, Cao Minh Dai, Bui Lan Anh, Pham Thi Anh Hong and Quach Ngo Diem Phuong

Abstract:
Background and Objective: Urena lobata L., a member of the Malvaceae family, is commonly used as a traditional medicine in tropical and subtropical regions. The leaf and root extracts of Urena lobata L. have been used to treat several diseases such as diabetes, rheumatism, gonorrhea and toothache. This study aims at improving culture conditions of Urena lobata L. hairy root for biomass and α-glucosidase inhibitory activity. Materials and Methods: Urena lobata L. hairy roots were induced by infecting the in vitro leaf explants with Agrobacterium rhizogenes ATCC 15834 and were grown in WPM liquid medium under darkness. Several factors affecting the growth of Urena lobata L. hairy roots including of inoculum sizes (0.05, 0.1, 0.15 and 0.2 g/30 mL FW), concentrations of sucrose (2, 3, 4 and 5%) and macroelements composition (half, full, double and triple-strength WPM macroelements) were investigated. The combitation of improved factors were compared with the original process for biomass and the inhibition of α-glucosidase. Results: The combitation of improved factors using WPM medium with double-strength of macroelements composition supplemented with 4% (w/v) sucrose, an inoculum size of 0.1 g/30 mL FW has higher biomass of hairy root (1.33 times) than the original process. In addition, the hairy root cultivated in combination culture has maintained α-glucosidase inhibitory activity (during 40 days) longer than in individually improved factors and original process. Conclusion: A protocol for Urena lobata L. hairy root culture was established which could produce a material have longer α-glucosidase inhibitory activity.
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How to cite this article:

Vu Thi Bach Phuong, Cao Minh Dai, Bui Lan Anh, Pham Thi Anh Hong and Quach Ngo Diem Phuong, 2019. Mass Propagation of Urena lobata L. Hairy Root Possessing α-Glucosidase Inhibitory Activity by Using Suitable Culture Conditions. Asian Journal of Plant Sciences, 18: 131-138.

DOI: 10.3923/ajps.2019.131.138

URL: https://scialert.net/abstract/?doi=ajps.2019.131.138

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