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Asian Journal of Plant Sciences

Year: 2018 | Volume: 17 | Issue: 4 | Page No.: 182-190
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Authors

Omaima A. Hussain

Hassan M. Sobhy

Amal Shawky Hathout

Amal Shawky Hathout

Ahmed Sayed Morsy Fouzy

Ahmed Sayed Morsy Fouzy

Keywords

Background and Objective: Fusarium sp. are considered one of the most important fungal genera; responsible for a broad range of plant diseases. The occurrence of Fusarium sp. in grains represents a problem in many countries around the world. Therefore, the aim of this work was to isolate and identify Fusarium sp. in several grains obtained from different Egyptian governorates by using the molecular technique. Materials and Methods: One hundred and fifty samples, 30 of each grain (wheat, white corn, yellow corn, feed corn, barley and rice) were obtained from different local markets from the following governorates; Cairo, Alexandria, Giza, Qena and Ghrbiya. Results: Data showed that all the grains were infested to various degrees with storage fungi. Fusarium sp., as well as several fungal species were isolated from different grains. Fusarium species were identified morphologically and then molecularly using polymerase chain reaction. The results revealed that the first strain exhibited a high level of 18S rRNA similarity (99%) with Fusarium verticillioides isolate (GenBank accession No. KJ207389.1), whereas, the second fungal strain of the sequenced 18S rRNA gene was identified as a close relative (99%) to Fusarium sp. (GenBank accession No. KJ190248.1). Conclusion: The partial or total sequencing of the 18S ribosomal DNA (rRNA) gene showed a fast technique for fungal classification.
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