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Asian Journal of Plant Sciences
  Year: 2018 | Volume: 17 | Issue: 2 | Page No.: 107-119
DOI: 10.3923/ajps.2018.107.119
Biomass and Flavonoid Production of Gynura procumbens (L.). Merr Adventitious Root Culture in Baloon-type Bubble-bioreactor Influenced by Elicitation
Hanik Faizah, Mulyadi Tanjung, Hery Purnobasuki and Yosephine Sri Wulan Manuhara

Abstract:
Background and Objective: Gynura procumbens (Lour.) Merr, an important medicinal plant, contains flavonoids that exhibit various pharmacological effects. Many strategies were used to increase the production of this valuable compound. The addition of biotic and abiotic elicitors is one of the most effective strategies for increasing the production of the bioactive compound. The aim of study is to investigate the effects of biotic (Saccharomyces cereviceae extract) and abiotic (CuSO4) elicitors on biomass and flavonoid content of G. procumbens adventitious root culture in balloon type bubble bioreactor. Methods: Adventitious roots were induced from leaf explants. Two gram adventitious roots were cultivated in 1 L balloon type bubble bioreactor containing 600 mL liquid MS medium supplemented with 5 mg L–1 IBA and 30 g L–1 sucrose. Then adventitious root growth determined based on fresh and dry weights that were harvested every 7 days up to 35 days of cultivation. Various concentrations of elicitors such as S. cereviceae extract (0.025, 0.05 and 0.1%) and CuSO4 (1, 3 and 5 mg L–1) were added on the 28th day to the culture. Adventitious root were harvested 7 day after elicitor treatment the flavonoid content is determined qualitatively by thin layer chromatography (TLC) and quantitatively with HPLC. Results: Various concentrations of S. cereviceae extract and CuSO4 affected the biomass and flavonoid production of G. procumbens adventitious roots. The optimal elicitation strategy was achieved with 0.025% S. cereviceae extract and 1 mg L–1 CuSO4. The treatment of 0.025% S. cereviceae extract resulted the highest biomass production and quercetin content reached 1.9-fold of the control culture. The treatment of 1 mg L–1 CuSO4 increased kaempferol content which was 13.3-fold of the control culture. Conclusion: Extract of S. Cereviceae and CuSO4 at low concentration can enhance production of biomass and flavonoid content of G. procumbens adventitious roots. Its condition can be used to develop production in large scale.
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How to cite this article:

Hanik Faizah, Mulyadi Tanjung, Hery Purnobasuki and Yosephine Sri Wulan Manuhara, 2018. Biomass and Flavonoid Production of Gynura procumbens (L.). Merr Adventitious Root Culture in Baloon-type Bubble-bioreactor Influenced by Elicitation. Asian Journal of Plant Sciences, 17: 107-119.

DOI: 10.3923/ajps.2018.107.119

URL: https://scialert.net/abstract/?doi=ajps.2018.107.119

 
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