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Asian Journal of Plant Sciences
  Year: 2015 | Volume: 14 | Issue: 2 | Page No.: 78-83
DOI: 10.3923/ajps.2015.78.83
 
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Comparison of Adventitious Shoot Formation of Garcinia mangostana via Embryogenesis and Direct Organogenesis

W.A. Qosim, R. Purwanto, G.A. Wattimena and Witjaksono

Abstract:
The adventitious shoot of mangosteen can be obtained in vitro from seed and leaf explants. This research was conducted to study effects of Benzyl Amino Purine (BAP) treatments on the formation of adventitious shoot of mangosteen in vitro via embryogenesis and direct organogenesis. Explants for embryogenesis were taken from cotyledon, where as those for direct organogenesis were from red young leaf of seedling. The BAP treatment of embryogenesis and direct organogenesis were 0.0, 11.1, 22.2, 33.3 and 44.4 μM, medium supplemented with 3% sugar, 0.8% agar, 1.39 μM PVP. Each experiment was arranged in a completely randomized design with BAP concentrations as treatments. The result of embryogenesis showed that MS medium supplemented with BAP 22.2 μM BAP produced the best effects on the percentage of explants forming adventitious shoot (53.7%), number of shoots (3.3), length of shoot (1.7 cm) and time to form shoot (17.3 days). The result of direct organogenesis showed that MS medium supplemented with BAP at a concentration of 2.2 μM resulted in the highest percentage of explants that formed shoot (39.8%), number of shoot per explants (1.3 shoot), number of pair leaf (1.2) and means number of shoot with the length 1-5 mm (1.3 shoot), 6-10 mm (0.8 shoot) and >10 mm (0.3 shoot). Furthermore, at the concentration tested, the shortest time to form shoot was 80.7 days. This highly efficient protocol of embryogenesis and direct organogenesis of mangosteen is needed for the improvement of mangosteen such as in genetic transformation, mutation breeding methods and propagation of mangosteen.
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How to cite this article:

W.A. Qosim, R. Purwanto, G.A. Wattimena and Witjaksono , 2015. Comparison of Adventitious Shoot Formation of Garcinia mangostana via Embryogenesis and Direct Organogenesis. Asian Journal of Plant Sciences, 14: 78-83.

DOI: 10.3923/ajps.2015.78.83

URL: https://scialert.net/abstract/?doi=ajps.2015.78.83

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