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Asian Journal of Plant Sciences
  Year: 2013 | Volume: 12 | Issue: 3 | Page No.: 97-108
DOI: 10.3923/ajps.2013.97.108
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Agrobacterium-mediated Transformation of Pearl Millet (Pennisetum typhoides (L.) R.Br.) for Fungal Resistance

S. Ignacimuthu and P. Kannan

A new Agrobacterium-mediated transformation system was developed for pearl millet using shoot apex explants, conferring resistance to leaf blast disease by inserting a rice chitinase (chi11) gene. Transgenic pearl millet lines (Pennisetum typhoides (L.) R.Br.) expressing rice chitinase gene with high levels of resistance to rust pathogen, Puccinia penniseti, were developed using Agrobacterium-mediated gene transfer method. The emryogenic calli derived from shoot apex of CO9 cultivar were transformed with LBA4404 (pSB1/pKAN-Rchit1.1) that harboured rice chitinase gene (chit11) under the control of maize ubiquitin (Ubi 1) promoter intron. Transgene (chit 11) in the middle of the T-DNA as used as probe in southern analysis. Out of six independent T0 plants tested for southern, three had single copy T-DNA insertions and three had two copies T-DNA insertions. All the six T0 plants carried complete T-DNA with the chitinase transgene. A segregation ratio of 3:1, reflecting T-DNA insertion at a single locus, was observed in the progeny of all the T0 plants which showed normal Mendelian pattern of transgene segregation. Western blot analysis of T1 plants revealed constitutive expression of chitinase at high levels. Bioassays of T1 plants indicated enhanced resistance to the rust pathogen, P. penniseti, in comparison to control plants. This is the first report on Agrobacterium-mediated transformation of pearl millet and first transgenic pearl millet with fungal resistance. This study underpins the introduction of numerous agronomically important genes into the genome of pearl millet in the future.
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How to cite this article:

S. Ignacimuthu and P. Kannan, 2013. Agrobacterium-mediated Transformation of Pearl Millet (Pennisetum typhoides (L.) R.Br.) for Fungal Resistance. Asian Journal of Plant Sciences, 12: 97-108.

DOI: 10.3923/ajps.2013.97.108






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