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Asian Journal of Plant Sciences
  Year: 2013 | Volume: 12 | Issue: 1 | Page No.: 46-50
DOI: 10.3923/ajps.2013.46.50
 
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Induced Mutation in Callus Cell Lines of Daucus carota L.

G.S. Nagananda, S. Rajath, Disha Agarwal, K. Ruby Mathew and S. Sundara Rajan

Abstract:
Daucus carota L. is an important vegetable food crop with high nutritive value. The changing climate, pollution, loss of vegetation has affected the crop quality and there is a need to improve the variety. So the present investigation was taken with an aim to induce mutation in callus cell lines for crop improvement. Callus induction from stem and leaf explants of in vitro grown Daucus carota L. plantlets was observed on 1X MS medium with 3% sucrose , 1% agar fortified with 2 mg L-1 2,4-D. Mutation was induced in Daucus carota L callus cell lines by sodium azide and UV radiations. Callus cell lines grown on media containing 200 μM of sodium azide gave the best result with callus multiplication. Callus exposed at different time intervals under UV radiation showed multiplication of callus in all the exposures. Shoot induction was achieved for both the mutants on 2 mg L-1 BAP containing medium. The physiological estimation revealed that protein content (0.460±0.04 mg mL-1) in 6 min UV exposed cell lines and carbohydrate content (0.820±0.08 mg mL-1) in 4 min UV exposed cell lines are high when compared to control cell lines, sodium azide and other UV mutated cell lines. Mutation was confirmed through DNA fingerprinting.
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How to cite this article:

G.S. Nagananda, S. Rajath, Disha Agarwal, K. Ruby Mathew and S. Sundara Rajan, 2013. Induced Mutation in Callus Cell Lines of Daucus carota L.. Asian Journal of Plant Sciences, 12: 46-50.

DOI: 10.3923/ajps.2013.46.50

URL: https://scialert.net/abstract/?doi=ajps.2013.46.50

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