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Asian Journal of Plant Sciences
  Year: 2013 | Volume: 12 | Issue: 1 | Page No.: 28-33
DOI: 10.3923/ajps.2013.28.33
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In vitro Callus Culture in Rauvolfia tetraphylla L.: Indole Alkaloid Production

S. Anitha and B.D. Ranjitha Kumari

Reserpine is an indole alkaloid reported to possess various biological activities and is chiefly used in chronic hypertension treatment for its hypotensive property and in the treatment of various psychological disorders for its tranquilizing effect. Reserpine is in great demand in the pharmaceutical industries for drug preparation and it has good export value. The quantity of this compound in intact plant varies with the source plant, place of its cultivation and the time of harvest. Hence the present study is undertaken with an objective of producing reserpine under controlled conditions in vitro by adjusting phytohormones concentration, sucrose concentration and light illumination duration. Various explants of Rauvolfia tetraphylla L. were tested for callusing and the reserpine content was analysed in the calli and the source plant. In the source plant, maximum amount of reserpine was found to present in the root (0.38 mg g-1 DW). Leaf derived callus was found to produce more amount of reserpine (0.9 mg g-1 DW) than calli derived from other explants, cultured on MS+B5 medium fortified with 9.04 μM 2,4-D and 4% sucrose with 16/8 hr light/dark illumination duration. This protocol can be used for commercial production of reserpine.
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How to cite this article:

S. Anitha and B.D. Ranjitha Kumari, 2013. In vitro Callus Culture in Rauvolfia tetraphylla L.: Indole Alkaloid Production. Asian Journal of Plant Sciences, 12: 28-33.

DOI: 10.3923/ajps.2013.28.33






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