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Asian Journal of Plant Sciences
  Year: 2013 | Volume: 12 | Issue: 5 | Page No.: 214-218
DOI: 10.3923/ajps.2013.214.218
 
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In vitro Regeneration of Four Ethiopian Varieties of Sesame (Sesamum indicum L.) using Anther Culture

M. Yifter, D.B. Sbhatu, F. Mekbib and E. Abraha

Abstract:
The study aimed at developing a suitable and reproducible protocol of in vitro regeneration of haploid sesame (Sesamum indicum L.) plantlets using anther culture. Anthers of four Ethiopian varieties, namely: Hirhir, Humera-1, Setit-1 and Non-Shatter were cultured in vitro to study their regenerating ability. Murashige and Skoog (MS) media supplemented with different concentrations and combinations of plant growth regulators (PGRs) were used. The highest callusing (56.20%) and callus weight (8.33 g) were observed in Hirhir cultured in MS medium supplemented with 2,4-D (2,4 dichlorophenoxyacetic acid) at 2.0 mg L-1 + BAP (benzylaminopurine) at 1.0 mg L-1. Shooting response was studied using green/friable calli of the varieties cultured in MS medium supplemented with BAP at the concentrations of 0.5, 1.0, 1.5 and 2.0 mg L-1 + NAA (naphthalene acetic acid) at 1.0 mg L-1. Calli of Hirhir cultured in MS medium supplemented with BAP at 2.0 mg L-1 + NAA at 1.0 mg L-1 were the best in terms of percentage shooting and number of days to shooting. Non-Shatter showed the weakest response to the same treatment. Rooting of shoots was studied with 0, 0.25, 0.5, 0.75 and 1.0 mg L-1 of IBA + 0.5 mg L-1 of NAA. In this case too, whereas Hirhir cultured in MS medium supplemented with 0.25 mg L-1 IBA (indole-3-butyric acid) +0.5 mg L-1 NAA showed the best response –87.15% in mean rooting, 11.66 mm in mean root length and 17.80 in mean days to rooting; Non-Shatter cultured in the same medium yield weakest response. Acclimatization responses of rooted in vitro seedlings were studied in coco peat and soil medium (comprising sand, cow dung and garden soil at a ratio of 2:1:1). Seedlings of Hirhir planted in both media gave better survival rate than that of the other varieties. The survival rates of Hirhir planted in coco peat and soil media were 66.7 and 50.0%, respectively.
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How to cite this article:

M. Yifter, D.B. Sbhatu, F. Mekbib and E. Abraha, 2013. In vitro Regeneration of Four Ethiopian Varieties of Sesame (Sesamum indicum L.) using Anther Culture. Asian Journal of Plant Sciences, 12: 214-218.

DOI: 10.3923/ajps.2013.214.218

URL: https://scialert.net/abstract/?doi=ajps.2013.214.218

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