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Asian Journal of Plant Sciences
  Year: 2013 | Volume: 12 | Issue: 3 | Page No.: 119-127
DOI: 10.3923/ajps.2013.119.127
 
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Influence of Nutrient Medium on Antioxidants Production of Date Palm (Phoenix dactylifera L.) Cultivars in vitro

Saleh M. Alturki, Wael F. Shehata and Mohammed I. Aldaej

Abstract:
Date palm (Phoenix dactylifera L.) is the major fruit crop in the Kingdom of Saudi Arabia. Presently, date palm is facing problem in the production of healthy in vitro callus tissues. The main objective of this study was to determine the influence of nutrient medium on antioxidants production of callus tissues from date palm cultivars in vitro. Four strengths of Murashige and Skoog (MS) culture medium (full, half, one quarter and three quarter strength) were used in the study. The MS strength significantly affected the vitality of the explants and its browning and ability for callus formation. The analysis of variance the cultivars and medium strength showed that they have a clear significantly effect on callus and browning formation of the explants. The highest total phenolic compounds (mg g-1) expressed as gallic acid equivalents induced per explant were 4.274 and 3.262 in Khalas and Shishi cultivars, respectively. The strength of the culture medium was inversely correlated with the antioxidant activity of plant extracts. In conclusion, for callus tissues from date palm cultivars, quarter strength MS medium offered a compromise between optimum growth in vitro and antioxidant phenolic accumulation.
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How to cite this article:

Saleh M. Alturki, Wael F. Shehata and Mohammed I. Aldaej, 2013. Influence of Nutrient Medium on Antioxidants Production of Date Palm (Phoenix dactylifera L.) Cultivars in vitro. Asian Journal of Plant Sciences, 12: 119-127.

DOI: 10.3923/ajps.2013.119.127

URL: https://scialert.net/abstract/?doi=ajps.2013.119.127

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