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Asian Journal of Plant Sciences
  Year: 2013 | Volume: 12 | Issue: 1 | Page No.: 1-10
DOI: 10.3923/ajps.2013.1.10
Laboratory Evaluation of Entomopathogenic Fungi Metarhizium anisophilae and Beauveria bassiana Against Termite, Macrotermes (Isoptera: Termitidae)
Addisu Sileshi, Waktole Sori and Mohamed Dawd

Abstract:
Termites, Macrotermes are major agricultural and domestic problem in Ethiopia causing serious damage with loss up to 100%. The use of synthetic termitecids was the most commonly used prevention measure to reduce the termites attack. However, these synthetic termiticides were known to be very harmful to the environment and non-target organisms. Therefore, efficacy of entompathogenic fungi, Metarhizium anisopliae (isolates PPRC-2 and MM) and Beauveria bassiana (isolates PPRC-56 and 9609) were evaluated against Macrotermes. The isolates were obtained from Ambo Plant Protection Research Center, Ethiopia. For each isolate concentrations of 1x105 to 1x109 conidia mL-1 were prepared and used as treatments. Untreated and standard (Diazinon 60% EC) checks were used for comparison. The treatments were laid out in Completely Randomized Design (CRD) and replicated thrice. The fungal isolates were evaluated by direct spraying of spore suspensions on worker Macrotermes spp. The result of the study revealed that all fungal isolates used were able to infect and cause mortality at all concentrations. The percent mortality of Macrotermes varied from 60 to 100% for M. anisopliae isolate MM at 1x105 to M. anisopliae isolate PPRC-2 at 1x109, respectively. Similarly, the percentage mortality of Macrotermes varied from 25-95% for B. bassiana isolate 9609 at low concentration and isolate PPRC-56 at highest concentration, respectively. The isolates had LT50 ranging from 7.74 days in M. anisopliae isolate PPRC-2 to 8.80 days in B. bassiana isolate 9609. The concentration response with the isolate PPRC-2 showed the lowest LC50 of 3.21x105 conidia mL-1 followed by isolates MM, PPRC-56 and 9609 with LC50 of 3.82x105, 4.39x105 and 5.08x105 conidia mL-1, respectively. In conclusions, the present study suggests that the use of entompopathogenic fungi, M. anisopilae and B. bassiana, at higher concentrations for seven days is an eco-friendly effective mycoinsecticides that causes more than 95% mortality of Macrotermes.
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How to cite this article:

Addisu Sileshi, Waktole Sori and Mohamed Dawd, 2013. Laboratory Evaluation of Entomopathogenic Fungi Metarhizium anisophilae and Beauveria bassiana Against Termite, Macrotermes (Isoptera: Termitidae). Asian Journal of Plant Sciences, 12: 1-10.

DOI: 10.3923/ajps.2013.1.10

URL: https://scialert.net/abstract/?doi=ajps.2013.1.10

 
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