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Asian Journal of Plant Sciences
  Year: 2010 | Volume: 9 | Issue: 3 | Page No.: 163-167
DOI: 10.3923/ajps.2010.163.167
Isolation of Lupeol, Stigmasterol and Campesterol from Petroleum Ether Extract of Woody Stem of Wrightia tinctoria
P.S. Jain and S.B. Bari

Abstract:
Aim of this study was to identify and characterize the bioactive principles from the woody stem of Wrightia tinctoria. For isolation of the compounds, the dried woody stem’s powder of Wrightia tinctoria was subjected to hot extraction with petroleum ether and subjected to chromatography. Three compounds (PEW-1, PEW-2 and PEW-3) were isolated and purified by chloroform. Mass spectrum of PEW-1, PEW-2 and PEW-3 showed a parent molecular ion [M+] peak at mlz 426 which corresponds to the molecular formula C30H50O, 412 corresponds to C29H48O and 400 corresponds to C28H48O. In the 1H-NMR spectrum of PEW-1, H-3 proton appeared as a triplet of a double doublet (tdd) at δ 3.21, H-29 proton gives two multiplates at δ 4.71 and δ 4.56, in lH-NMR spectrum of PEW-2, H-3 proton appeared as a triplet of a double doublet (tdd) at δ3.62 and H-6 olefinic proton showed a multiplet at δ5.14. Two olefenic protons appeared downfield at δ 4.16 (m) and δ 4.14 (m). Six methyl proton appeared at δ 1.27, δ 1.19, δ 1.07, δ 1.00, δ 0.98 and δ 0.91 for methyl group and in the 1H-NMR data of PEW-3, H-3 proton appeared at δ 3.21 as a triplet of a double doublet H-6 olefinic proton showed a multiplet at δ 5.10 and Six methyl proton appeared at δ 1.27, δ 1.14, δ 1.09, δ 1.00, δ 0.98 and δ 0.95 for methyl group. From the physical, chemical and spectral characteristics, PEW-1, PEW-2 and PEW-3were concluded as lupeol, stigmasterol and campetosterol.
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How to cite this article:

P.S. Jain and S.B. Bari, 2010. Isolation of Lupeol, Stigmasterol and Campesterol from Petroleum Ether Extract of Woody Stem of Wrightia tinctoria. Asian Journal of Plant Sciences, 9: 163-167.

DOI: 10.3923/ajps.2010.163.167

URL: https://scialert.net/abstract/?doi=ajps.2010.163.167

 
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