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Asian Journal of Plant Sciences
  Year: 2009 | Volume: 8 | Issue: 4 | Page No.: 285-292
DOI: 10.3923/ajps.2009.285.292
 
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Expression Pattern of GS3 During Panicle Development in Rice under Drought Stress: Quantification Normalized Against Selected Housekeeping Genes in Real-Time PCR

Jia-Liang Zhang, Dong-Hua Liu, Zhi-Hua Wang, Can Yu, Jin-Hua Cao, Chun-Tai Wang and De-Ming Jin

Abstract:
Many studies on mechanisms under drought stress have been based on gene expression. Quantitative Real-Time PCR (QRT-PCR) is the most sensitive method to detect the transcript of interest gene. To avoid bias, expression of the target gene is usually normalized relative to one or multiple reference genes which should not fluctuate in all samples. The most widely used reference genes are those which belong to actin family; however, many researchers make use of paralogous genes for actin without proper validation of their presumed stability of expression. If an unstable housekeeping gene (HKG) is selected to normalize the target, the results obtained may be spurious. So, we evaluated the gene expression of 10 HKGs, including 8 paralogous genes for actin, together with b-tub and eEF-1a in rice. For the entire sample pools analyzed, ACT(X16280)1 and eEF-1a were the most stable genes in the different stages of rice panicle development. In this study, we used ACT(X16280)1, eEF-1a and the geometric mean of ACT(X16280)1 and eEF-1a as internal controls to study the relative expression of gene GS3, controlling grain length and weight, during different stages of panicle development under drought stress. A sharp increase of GS3 expression (up to 4.8 fold) at spikelet primordium differentiation stage was observed under drought stress. The shrinking of grain size under drought stress could be attributed to the over expression of GS3 which is a negative regulator for grain size.
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How to cite this article:

Jia-Liang Zhang, Dong-Hua Liu, Zhi-Hua Wang, Can Yu, Jin-Hua Cao, Chun-Tai Wang and De-Ming Jin, 2009. Expression Pattern of GS3 During Panicle Development in Rice under Drought Stress: Quantification Normalized Against Selected Housekeeping Genes in Real-Time PCR. Asian Journal of Plant Sciences, 8: 285-292.

DOI: 10.3923/ajps.2009.285.292

URL: https://scialert.net/abstract/?doi=ajps.2009.285.292

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