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Asian Journal of Plant Sciences

Year: 2009 | Volume: 8 | Issue: 2 | Page No.: 111-119
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Authors

Z.E. Pu

Y.C. Hou

X.X. Xu

Z.H. Yan

Y.M. Wei

X.J. Lan

Y.L. Zheng

Keywords

Research Article

Genetic Diversity among Barley Populations from West China Based on RAMP and RAPD Markers

Z.E. Pu, Y.C. Hou, X.X. Xu, Z.H. Yan, Y.M. Wei, X.J. Lan and Y.L. Zheng
Using RAMP and RAPD for detecting the genetic diversity of 46 barley accessions, collected from west China. Seventeen primer combinations produced 104 discernible RAMP fragments of which 96 (91%) were polymorphic. The number of fragments per primer combination varied from 2 to 11, with the mean of 6.18. The mean of Polymorphism Information Content (PIC) for the RAMPs was 0.752. On the basis of 96 polymorphic fragments, each genotype had a unique banding profile and the Genetic Similarity (GS) coefficient varied between 0.450 and 0.960, with the mean of 0.803. In RAPD analysis, 28 out of 43 bands (65%) were polymorphic. The number of alleles ranged from 1 to 8, with an average of 2.53 per primer. The mean of Polymorphism Information Content (PIC) for the RAPDs was 0.282. The Genetic Similarity (GS) coefficient varied between 0.679 and 1.000, with the mean of 0.898. The mean values of GS within the HS, HA and HV groups were 0.909, 0.893 and 0.913, respectively. RAMP-based genetic similarity matrices were compared with the corresponding RAPD-based matrices by the Mantel test. A poor of correlation was found between both sets of data, indicating little relationship between these two estimators of genetic similarity. The cluster results based on RAMP more faithfully distinguished the experimental accessions than did the RAPD results. Both dendrograms generated by the RAPD matrix and the RAMP matrix all agree better with the groups of the genotypes, but the dendrogram generated by the RAPD matrix agrees better with the geographic origins of the genotypes than the dendrogram generated by the RAMP results.
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