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Asian Journal of Plant Sciences
  Year: 2008 | Volume: 7 | Issue: 7 | Page No.: 677-681
DOI: 10.3923/ajps.2008.677.681
 
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Callus Induced Organogenesis in Okra (Abelmoschus esculents L. Moench.)

M. Anisuzzaman, S. Jarin, K. Naher, M.M. Akhtar, M.J. Alam, M. Khalekuzzaman, I. Alam and M.F. Alam

Abstract:
A viable protocol has been developed for indirect shoot organogenesis of okra. To establish a stable and high-frequency plant regeneration system, leaf disc and hypocotyl explants were tested with different combinations of α-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA),iIndole-3-butyric acid (IBA), thidiazuron (TDZ) and 6-benzylaminopurine (BAP). Morphogenic callus induction was observed in highest frequency from hypocotyl explant by culturing in MS medium supplemented with 2.0 mg L-1 NAA plus 0.5 mg L-1 TDZ. The highest percentage of shoot regeneration and highest mean number of shoot per callus mass was obtained with 2.0 mg L-1 BAP plus 0.1 mg L-1 IBA. Root formation was observed from callus induced in medium containing 1.5 mg L-1 NAA. Morphogenic difference due to explant type is clear for the studied in vitro traits. About 80% of regenerated plantlets were survived and showed new leaves development under ex vitro condition. This protocol would be useful to create somaclonal variation and to utilize transgenic approach for varietal improvement of okra.
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How to cite this article:

M. Anisuzzaman, S. Jarin, K. Naher, M.M. Akhtar, M.J. Alam, M. Khalekuzzaman, I. Alam and M.F. Alam, 2008. Callus Induced Organogenesis in Okra (Abelmoschus esculents L. Moench.). Asian Journal of Plant Sciences, 7: 677-681.

DOI: 10.3923/ajps.2008.677.681

URL: https://scialert.net/abstract/?doi=ajps.2008.677.681

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