Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Asian Journal of Plant Sciences
  Year: 2008 | Volume: 7 | Issue: 7 | Page No.: 660-665
DOI: 10.3923/ajps.2008.660.665
In vitro Propagation of Gymnema sylvestre
C. Subathra Devi and V. Mohana Srinivasan

Abstract:
The need of MS salts for shoot sprouting and proliferation shows the high salt requirement for the growth of Gymnema sylvestre. Influence of plant growth regulators, IAA, BA, 2,4-D and kinetin on shoot sprouting was investigated. Synergistic effect of Vitamin B2 was studied. To overcome phenolic exudation and the effects of antioxidants, the effect of activated charcoal, citric acid and ascorbic acid were investigated. Incorporation of citric acid (100 mg L-1) to the medium prevented phenolic exudation and increased the production of healthy normal shoots and shoot bud differentiation in Gymnema sylvestre. There was a considerable improvement in rooting as about 53% shoots could be induced to root on 1/2 MS medium within 45 days with a fairly good length and number of roots per shoot. MS medium containing 1 mg L-1 BA+0.5 mg L-1 IAA + 100 mg L-1 Vitamin B2+100 mg L-1 citric acid is best for shoot proliferation and 1/2 strength MS medium with IBA 3 mg L-1 is best for root induction.
 [Fulltext PDF]   [Fulltext HTML]   [XML: Abstract + References]   [References]   [View Citation]  [Report Citation]
 RELATED ARTICLES:
  •    Evaluation of Cotyledonary Node of Clitoria ternatea L. for High Frequency in vitro Axillary Shoot Proliferation
How to cite this article:

C. Subathra Devi and V. Mohana Srinivasan, 2008. In vitro Propagation of Gymnema sylvestre. Asian Journal of Plant Sciences, 7: 660-665.

DOI: 10.3923/ajps.2008.660.665

URL: https://scialert.net/abstract/?doi=ajps.2008.660.665

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 

Curve Bottom