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Asian Journal of Plant Sciences

Year: 2008 | Volume: 7 | Issue: 2 | Page No.: 177-182
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Authors

A. Shekafandeh

M. Khosh-Khui

Keywords

Research Article

Effects of Bud Position and Culture Medium on Shoot Proliferation from Nodal Culture of Two Mature Guava Cultivars

A. Shekafandeh and M. Khosh-Khui
In this research, the rate of shoot proliferation from different nodes in different positions on shoot [uppermost node (N1), the second node (N2) and the third node (N3)] from two mature guava cultivars, white fleshed Local-1 and red fleshed Local-2 on different culture media (MS and WPM) with different BA concentrations was investigated. The results showed that in both cultivars, the best BA concentration was 1.5 mg L-1. There was a significant interaction between bud positions, culture media and cultivars on shoot proliferation and shoot length. In Local-1 maximum shoot number (2.86) was recorded from buds in N2 position on MS medium followed by 2.43 shoots/explant from buds in N2 positions on WPM medium. In Local-2 on both medium the buds in N1 position had significantly a higher number of shoots/explant (1.57 on MS and 1.86 on WPM) than the buds in N3 positions (0.43 on MS and 0.71 on WPM). Shoot length, had a similar trend.
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