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Asian Journal of Plant Sciences

Year: 2007 | Volume: 6 | Issue: 5 | Page No.: 757-764
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Authors

Irving V. Occena

Elmer-Rico E. Mojica

Florinia E. Merca

Keywords

Research Article

Isolation and Partial Characterization of a Lectin from the Seeds of Artocarpus camansi Blanco

Irving V. Occena, Elmer-Rico E. Mojica and Florinia E. Merca
The aim of this study is to isolate lectin from a seed which had no economical use. A lectin was isolated and purified from the seeds of Artocarpus camansi Blanco by extraction with phosphate buffered saline at pH 7.2. Further purification was done by precipitation with ammonium sulfate at 0-90% saturation. The dialyzed ammonium sulfate precipitate was subjected to gel filtration chromatography using Sephadex G-200 and fractions were tested for lectin activity. Native PAGE was employed to establish the homogeneity of the lectin positive fractions. Fractions with the same band were pooled and lyophilized. The purified lectin is non-blood type specific since it agglutinated all human blood types. It is blood group specific because it agglutinated blood from cow but not from swine. The purified lectin is a complete lectin since it agglutinated erythrocytes without trypsin treatment and addition of metal ions. However, trypsination of erythrocytes increases the agglutination activity. The isolated lectin did not show any specificity towards the ten sugars used in this study. The molecular weight of the purified lectin was estimated by using Sephadex G-200 and was found to be around 2,000 kDa since it eluted in the void volume. The lectin was found to be stable at 20-40°C.
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