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Asian Journal of Plant Sciences
  Year: 2007 | Volume: 6 | Issue: 8 | Page No.: 1235-1240
DOI: 10.3923/ajps.2007.1235.1240
 
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In vitro Conservation of Cleisostoma areitinum (Rchb.f.) Garay, Rare Thai Orchid Species by an Encapsulation-Dehydration Method
P. Maneerattanarungroj, S. Bunnag and M. Monthatong

Abstract:
Protocorm cryopreservation has been performed for ex situ preservation of Cleisostoma areitinum (Rchb.f.) Garay, rare Thai orchid species. Cryopreservation of protocorms was investigated by an encapsulation-dehydration method. The highest regrowth percentage of cryopreserved explants was observed when they were precultured on semi-solid ND medium with 0.25 M sucrose in the dark for 1 week then transferred to liquid ND medium containing 0.75 M sucrose for 2 days. The treated explants were dehydrated with silica gel in laminar air-flow for 5 h prior to immersion in liquid nitrogen for 1 h. Protocorm viability was tested by the TTC (2, 3, 5-triphenyltetrazoliumchloride) assay and regrowth ability was assessed by determining the survival percentage after recovery for 2 weeks. The survival rate of cryopreserved protocorms was 49% while percentage of TTC assay was 77%. Consequently, the simple and reliable protocol for cryopreservation of C. areitinum was clearly revealed in this study.
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How to cite this article:

P. Maneerattanarungroj, S. Bunnag and M. Monthatong, 2007. In vitro Conservation of Cleisostoma areitinum (Rchb.f.) Garay, Rare Thai Orchid Species by an Encapsulation-Dehydration Method. Asian Journal of Plant Sciences, 6: 1235-1240.

DOI: 10.3923/ajps.2007.1235.1240

URL: https://scialert.net/abstract/?doi=ajps.2007.1235.1240

 
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