Research Article

In vitro Conservation of Cleisostoma areitinum (Rchb.f.) Garay, Rare Thai Orchid Species by an Encapsulation-Dehydration Method

P. Maneerattanarungroj, S. Bunnag and M. Monthatong

Protocorm cryopreservation has been performed for ex situ preservation of Cleisostoma areitinum (Rchb.f.) Garay, rare Thai orchid species. Cryopreservation of protocorms was investigated by an encapsulation-dehydration method. The highest regrowth percentage of cryopreserved explants was observed when they were precultured on semi-solid ND medium with 0.25 M sucrose in the dark for 1 week then transferred to liquid ND medium containing 0.75 M sucrose for 2 days. The treated explants were dehydrated with silica gel in laminar air-flow for 5 h prior to immersion in liquid nitrogen for 1 h. Protocorm viability was tested by the TTC (2, 3, 5-triphenyltetrazoliumchloride) assay and regrowth ability was assessed by determining the survival percentage after recovery for 2 weeks. The survival rate of cryopreserved protocorms was 49% while percentage of TTC assay was 77%. Consequently, the simple and reliable protocol for cryopreservation of C. areitinum was clearly revealed in this study.

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P. Maneerattanarungroj, S. Bunnag and M. Monthatong, 2007. In vitro Conservation of Cleisostoma areitinum (Rchb.f.) Garay, Rare Thai Orchid Species by an Encapsulation-Dehydration Method. Asian Journal of Plant Sciences, 6: 1235-1240.

DOI: 10.3923/ajps.2007.1235.1240

URL: https://scialert.net/abstract/?doi=ajps.2007.1235.1240

Asian Journal of Plant Sciences

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P. Maneerattanarungroj

S. Bunnag

M. Monthatong

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Research Article

In vitro Conservation of Cleisostoma areitinum (Rchb.f.) Garay, Rare Thai Orchid Species by an Encapsulation-Dehydration Method

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