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Asian Journal of Plant Sciences
  Year: 2006 | Volume: 5 | Issue: 4 | Page No.: 623-629
DOI: 10.3923/ajps.2006.623.629
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Regeneration of (Vicia faba L.) Cultivars from Mature Seed Cotyledons

M.A. Hamdy Aly and Kazumi Hattori

The objective of this research was to develop recipient system of faba bean cultivars (Vicia faba L.) and to obtain 686 Sudan; 1059 Morocco; NEB 367; ILB 752; 724 Norway; 1083 Australia; Wasa soramame and Cairo 241 plants through direct regeneration in vitro. The morphogenetic of seeds in selected faba bean cultivars (Vicia faba L.) was realized via one way: direct regeneration of plants from mature cotyledons. The cotyledons with ten combinations of growth regulators: (Kin.: 6-Furfurylaminopurine N6-Furfuryladenine C10H9N5O; BAP: 6-Benzylamino-9-(2-tetrahydropyranyl)-9H-purine C17H19N5O; TDZ: N-phenyl-N`-1,2,3-thiadiazol-5-ylurea; NAA: α-Naphthaleneacetic acid 1-Naphthylacetic acid C12H10O2 and IBA 4-(3-Indolyl) butanoic acid C12H13NO2) were compared. Effect of growth regulator treatments and cotyledon of cultivars were generally small within inter-cultivars, but callus and shoot formation different intra-cultivars. The other cultivars generally did not produce calli. The developed in vitro systems of faba bean cultivars (Vicia faba L.) plants approve us to use them for different purposes of plant biotechnology: genetic transformation, multiplication and conservation in vitro of valuable (Vicia faba L.).
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How to cite this article:

M.A. Hamdy Aly and Kazumi Hattori , 2006. Regeneration of (Vicia faba L.) Cultivars from Mature Seed Cotyledons. Asian Journal of Plant Sciences, 5: 623-629.

DOI: 10.3923/ajps.2006.623.629






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