Abstract:
Three varieties of water chestnut (Trapa quadrispinosa, T. bispinosa and T. acornis) collected from Rajshahi, Naogaon, Natore have been used in this study. Embryonal explants were found to be best for callus induction on the medium containing half strength MS salts in combination of 2.5 mg L-1 2,4-D and 1 mg L-1 BA inall three varieties. For survival of explant 0.1% HgCl2 having 7 minutes of treatment period was found to be best. Addition of CH (2.0 mg L-1) and PG (2.0 mg L-1) proved to be effective to overcome the phenolic oxidant during callus induction. Callus derived plantlet formation was being a difficult task in water chestnut. Liquid half strength MS medium supplemented with 1.1 mg L-1 BA and 0.5 mg L-1 NAA has been proved to be best for shoot proliferation in all the varieties. Moreover, addition of 0.5 mg L-1 GA3 with aforesaid combination highly stimulated the axillary shoot elongation of Trapa sp. Rooting of produced shoot showed highest percentage in a liquid half strength MS medium containing 1.1 mg L-1 IBA. This in vitro plantlet were then transferred and acclimatized in plastic pots under favorable humidity and temperature. After 4 weeks of acclimatization callus derived plantlets were established successfully in a water chestnut field. It was found that all the plantlets were disease free and the survival rate was almost 95%.
A. Hoque , A. Nahar , M.A. Razvy , M.K. Biswas and A.H. Kabir , 2006. Micropropagation of Water Chestnut (Trapa sp.) Through Local Varieties of Rajshahi Division. Asian Journal of Plant Sciences, 5: 409-413.
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