Research Article

In vitro Adventitious Shoot Formation and Organogenesis from Embryonic Axes of Myrica rubra Sieb. and Zucc., (Red Bayberry)

Syed Asghar, Ligeng Chen, Xinhua He and Yonghua Qin

In the present study an efficient in vitro adventitious shoot formation and organogenesis protocol was developed for Myrica rubra. Embryonic axes were excised and cultured on MS medium fortified with 0.6 mg L^-1 TDZ for different time intervals. Explants not exposed to TDZ concentration did not germinate. Exposure of explants for 2-3 weeks was found the optimal time interval for induction of adventitious shoots. Shoot regeneration was suppressed by longer exposures. Highest shoot induction frequency (99.80%) was observed when explants were exposed for 21 days followed by 14 days (97.00%) exposure. Maximum number of shoots (16.66) were recorded when explants were exposed to TDZ containing media for 21 days. Shoots were tremendously elongated with proper regulation of BA concentrations alone or in combination with NAA. Longer shoots (4.60 cm) were harvested with 0.5 mg L^-1 BA in combination with 0.9 mg L^-1 NAA. Roots were induced with a high frequency of 100% in MS medium supplemented with 0.6 mg L^-1 IBA. Plants were successfully acclimatized to the green house conditions.

PDF Fulltext XML References Citation

How to cite this article

Syed Asghar, Ligeng Chen, Xinhua He and Yonghua Qin, 2005. In vitro Adventitious Shoot Formation and Organogenesis from Embryonic Axes of Myrica rubra Sieb. and Zucc., (Red Bayberry). Asian Journal of Plant Sciences, 4: 356-360.

DOI: 10.3923/ajps.2005.356.360

URL: https://scialert.net/abstract/?doi=ajps.2005.356.360

Asian Journal of Plant Sciences

Article Trend

Total views 273

Search

Authors

Syed Asghar

Ligeng Chen

Xinhua He

Yonghua Qin

Keywords

Research Article

In vitro Adventitious Shoot Formation and Organogenesis from Embryonic Axes of Myrica rubra Sieb. and Zucc., (Red Bayberry)

How to cite this article

Leave a Comment