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Asian Journal of Plant Sciences
  Year: 2004 | Volume: 3 | Issue: 2 | Page No.: 231-238
DOI: 10.3923/ajps.2004.231.238
 
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Genetic Diversity of Echinohloa Crus-galli Var. Crus-galli (L.) Beauv (Barnyardgrass: Poaceae) Ecotypes in Malaysia and Indonesia as Revealed by RAPD Markers

Arifin Tasrif, Abdul ShukorJuraimi , Jugah Kadir , Soetikno S. Sastroutomo and Suhaimi Napis

Abstract:
Fourty ecotypes of Echinochloa crus-galli Var. crus-galli (Barnyardgrass: Poaceae) collected from Malaysian (26 ecotypes) and Indonesian (14 ecotypes) rice fields were studied using Random Amplified Polymorphic DNA (RAPD) markers. The ecotypes were collected randomly from 11 locations in Malaysia and 6 locations in Indonesia. Individual ecotypes from all the sites were clearly distinguished from each other by RAPD-PCR polymorphisms. The 26 individual ecotypes of E. crus-galli Var. crus-galli were clustered into four groups at a similarity level of 60% in UPGMA (unweighted pair group method with arithmetic averages). Four groups were identified of the 14 barnyardgrass ecotypes from Indonesia with similarity level of 64%. Six groups have been classified among Malaysian and Indonesian ecotypes of barnyardgrass. Group one had the highest number in the phylogenetic majority, while group 4 and 6 had one ecotype, respectively. The results showed that the Malaysian ecotypes were more variable than Indonesia ecotypes. Three Malaysian ecotypes (in group five and six) formed different clusters. Two Malaysia ecotypes had genetic relationship with 4 Indonesia ecotypes (group two), while the rest of the ecotypes correlated with the geographic distribution. RAPD-PCR markers revealed relatively low genetic variation between barnyardgrass ecotypes. Thus, the genetic diversity observed might be attributed to the diversity among individual ecotypes from divergent locations that may affect weed management systems especially herbicide application. The genetic differences within and among barnyardgrass ecotypes were not sufficient enough for biocontrol implications.
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How to cite this article:

Arifin Tasrif, Abdul ShukorJuraimi , Jugah Kadir , Soetikno S. Sastroutomo and Suhaimi Napis , 2004. Genetic Diversity of Echinohloa Crus-galli Var. Crus-galli (L.) Beauv (Barnyardgrass: Poaceae) Ecotypes in Malaysia and Indonesia as Revealed by RAPD Markers. Asian Journal of Plant Sciences, 3: 231-238.

DOI: 10.3923/ajps.2004.231.238

URL: https://scialert.net/abstract/?doi=ajps.2004.231.238

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