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Asian Journal of Plant Sciences
  Year: 2002 | Volume: 1 | Issue: 5 | Page No.: 585-587
DOI: 10.3923/ajps.2002.585.587
Cowpea Germplasm Evaluation for Virus Resistance under Greenhouse Conditions
Muhammad Bashir, Zahoor Ahmad and Abdul Ghafoor

Abstract:
In order to identify sources of resistance in cowpea against blackeye cowpea mosaic virus (BLCMV), two hundred cowpea germplasm accessions were evaluated under greenhouse conditions. Ten to 15 plants of each accession were artificially inoculated by sap prepared from virus-infected plants. Disease severity on each line was recorded at 15 days interval. The symptomless plants were tested by indirect enzyme-linked immunosorbent assay (ELISA) to separate the resistant plants from susceptible ones. Only two accessions (27168 and 27192) out of 134 of local origin were found resistant to BlCMV, whereas 23 (34.8%) were resistant out of 66 exotic lines. Twenty genotypes expressed segregation pattern towards disease reaction and the others were susceptible. In this study some new sources of resistance have identified which can be used to breed to breed virus-resistant cowpea cultivars.
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How to cite this article:

Muhammad Bashir, Zahoor Ahmad and Abdul Ghafoor , 2002. Cowpea Germplasm Evaluation for Virus Resistance under Greenhouse Conditions. Asian Journal of Plant Sciences, 1: 585-587.

DOI: 10.3923/ajps.2002.585.587

URL: https://scialert.net/abstract/?doi=ajps.2002.585.587

 
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