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Asian Journal of Plant Pathology
  Year: 2020 | Volume: 14 | Issue: 1 | Page No.: 27-35
DOI: 10.3923/ajppaj.2020.27.35
 
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Surfactin Production, Biofilm Formation and Antagonistic Activity Against Phytopathogens by Bacillus velezensis

Athidtaya Kumvinit and Angsana Akarapisan

Abstract:
Background and Objective: Bacillus spp. have a wide range of activities in the biocontrol potential against various phytopathogens. This study was focused to identify the antagonistic bacteria and to evaluate for their efficacy against the bacterial pathogens of potato. Materials and Methods: Bacillus sp. strains BB06, BB10, CF58 and CR74 were previously isolated from the rhizosphere. They were identified by 16S rRNA gene and gyrA gene. The strains were tested for hydrolytic enzyme and plant growth-promoting activities. Also, the strains were analyzed the surfactin antibiotic by LCMS-QTOP. Results: Based on the 16S rRNA gene and gyrA gene sequence analyses, the strains were identified as Bacillus velezensis. All strains showed a broad inhibitory spectrum and produced zones of inhibition against bacterial pathogens of potato, included Pectobacterium carotovorum subsp. brasiliense, Dickeya dadantii, Bacillus altitudinis and Ralstonia solanacearum. The results of in vitro assays showed that all isolates can produce protease, amylase, cellulase, lipase, IAA (auxin), siderophores and phosphate solubilization. Further analysis of the surfactin antibiotic by LCMS-QTOP, revealed the characteristic molecular ions mode [M-H-] with an m/z around 1034. The strains showed analogous biofilm formation to support the biocontrol properties. Conclusion: B. velezensis strain BB06, BB10, CF58 and CR74 can be recommended as potential biocontrols.
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How to cite this article:

Athidtaya Kumvinit and Angsana Akarapisan, 2020. Surfactin Production, Biofilm Formation and Antagonistic Activity Against Phytopathogens by Bacillus velezensis. Asian Journal of Plant Pathology, 14: 27-35.

DOI: 10.3923/ajppaj.2020.27.35

URL: https://scialert.net/abstract/?doi=ajppaj.2020.27.35

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