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Asian Journal of Plant Pathology

Year: 2017 | Volume: 11 | Issue: 2 | Page No.: 95-101
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Authors

Angsana Akarapisan

Angsana Akarapisan

Athidtaya Kumvinit

Soraya Ruamrungsri

Keywords

Research Article

Bulb Rot of Amaryllis Caused by Sclerotium rolfsii and Effect of Fungicides on in vitro Inhibition of Mycelial Growth

Angsana Akarapisan Angsana Akarapisan's LiveDNA, Athidtaya Kumvinit and Soraya Ruamrungsri
Background and Objective: Amaryllis production has dropped due to bulb rot in the cropping areas of Chiang Mai, Thailand. It has become a recurrent problem of amaryllis bulb production. It is very difficult to develop effective management strategies for soil-borne pathogens that produce sclerotia because of their long-term survival in the soil. Fungicides are among the most effective options for the control of diseases caused by soil-borne pathogens. The objectives of this study were to identify the pathogen of amaryllis bulb rot and to evaluate fungicides for their efficacy against the pathogen. Methodology: The experiments identified the pathogen based on morphological characteristics and PCR analysis using specific primers. The pathogenicity of fungal isolates was tested in the greenhouse and compared with the non-treated control. Five fungicides including captan, copper oxychloride, mancozeb, carbendazim and etridiazole+quintozene were tested by the poison agar method, this analysis was arranged in a completely randomized design with 5 replicates of each treatment. Results: The initial disease symptom was water-soaked lesions in bulbs followed by total rotting of bulbs and leaf sheath blight. White cottony mycelia formed on the blubs of diseased plants. Clamp connections were common at every septum. The isolate was identified as Sclerotium rolfsii. A pathogenicity test was conducted in the greenhouse, where the symptoms of leaf sheath blight were observed. Also, bulb fresh weight, circumference and number of bulblets were decreased after pathogen inoculation. Effective and efficient integrated management of crop diseases often includes the use of fungicides. At recommended doses, etridiazole+quintozene, captan and mancozeb significantly reduced the growth of the fungus by 100, 68.85 and 64.56%, respectively. Conclusion: The results revealed that S. rolfsii was the causal agent of bulb rot of amaryllis in Northern Thailand. The most effective fungicide treatment against S. rolfsii from rotted amaryllis bulbs was etridiazole+quintozene.
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Angsana Akarapisan, Athidtaya Kumvinit and Soraya Ruamrungsri, 2017. Bulb Rot of Amaryllis Caused by Sclerotium rolfsii and Effect of Fungicides on in vitro Inhibition of Mycelial Growth. Asian Journal of Plant Pathology, 11: 95-101.

URL: https://scialert.net/abstract/?doi=ajppaj.2017.95.101

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