Expression of Class II Chitinase Gene in Chilli (Capsicum annuum L.) as Response to Fusarium oxysporum Pathogen Attack

Research Article

**Expression of Class II Chitinase Gene in Chilli (Capsicum annuum L.) as Response to Fusarium oxysporum Pathogen Attack**

Rejeki Siti Ferniah, Rina Sri Kasiamdari, Achmadi Priyatmojo and Budi Setiadi Daryono

Chilli plants have many mechanism defenses to pathogen attack. The biotic stress can induce genes encoding Pathogenesis Related (PR) proteins to increase expression. The aim of this research is to analyze a molecular responds of CaChi2 gene as PR protein in chilli, which is infected by fungal pathogenic Fusarium oxysporum. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the fluorescence and the ddCt method was used to analyze the expression level of CaChi2 in chilli. The result showed, up-regulated expression of resistant plants but no change in expression of susceptible plants after many range of time. It is concluded that the CaChi2 gene can be a molecular marker of resistance to Fusarium oxysporum in chilli.

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Rejeki Siti Ferniah, Rina Sri Kasiamdari, Achmadi Priyatmojo and Budi Setiadi Daryono, 2015. Expression of Class II Chitinase Gene in Chilli (Capsicum annuum L.) as Response to Fusarium oxysporum Pathogen Attack. Asian Journal of Plant Pathology, 9: 142-147.

URL: https://scialert.net/abstract/?doi=ajppaj.2015.142.147

Asian Journal of Plant Pathology

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Authors

Rejeki Siti Ferniah

Rina Sri Kasiamdari

Achmadi Priyatmojo

Budi Setiadi Daryono

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Research Article

**Expression of Class II Chitinase Gene in Chilli (Capsicum annuum L.) as Response to Fusarium oxysporum Pathogen Attack**

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