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Asian Journal of Plant Pathology
  Year: 2013 | Volume: 7 | Issue: 2 | Page No.: 60-73
DOI: 10.3923/ajppaj.2013.60.73
Differentiation Between Glomus Species in Egyptian Soil Using Fatty Acid Methyl Ester Profiles
K.K. Sabet, M.S. Mansour, S.A. El-Hadad, Abeer M. Shaltout and Sozan E. El-Abeid

Abstract:
The present study was to investigate the variation among Glomus species. Standard method used for Extraction, purification of Vesicular Arbuscular Mycorrhizal (VAM) fungi, during summer and winter season. Study Effect of some factors on number of spores collected from the rhizosphere of various plants in Egypt. Used single spores technique inoculated on maize root to purified and identified isolates of Glomus spp. The highest number of spores was found in summer season while the lowest number was found in winter season. Variation of Glomus spp. isolates among polymorphic DNA markers was studied. Species 2, 3, 4 and 7 identified by VANS1/VALETC primer as Glomus etunicatum. Fatty Acid Methyl Ester (FAME) profiles were analyzed to assess the diversity and quantity of fatty acids in 8 isolates of Glomus species. Spores and endomycorrhizal roots of Sudan grass (Sorghum sudanense) were examined. Analysis of lipid for species found that affinity specific and generic levels. Differentiation on the genus level using lipid profiles and content which was carried out by evaluating the methyl esters of fatty acids, revealed convergence between studied isolates. Palmitic acid (16:0), Oleic acid (cis18:1n9) and Stearic acid (18:0) were the dominant fatty acid of the Glomus spp., while the dominant fatty acids of Glomus etunicatum isolates were myristic acid (14:0) palmitoleic acid (16:1n7 cis), archidic acid (20:0), palmitic acid (16:0), oleic acid (cis18:1n9) and stearic acid (18:0). This study indicates that using lipids as biochemical markers to identify the different genus of AM fungi and differentiate between the species.
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How to cite this article:

K.K. Sabet, M.S. Mansour, S.A. El-Hadad, Abeer M. Shaltout and Sozan E. El-Abeid, 2013. Differentiation Between Glomus Species in Egyptian Soil Using Fatty Acid Methyl Ester Profiles. Asian Journal of Plant Pathology, 7: 60-73.

DOI: 10.3923/ajppaj.2013.60.73

URL: https://scialert.net/abstract/?doi=ajppaj.2013.60.73

 
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