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American Journal of Plant Physiology
  Year: 2018 | Volume: 13 | Issue: 1 | Page No.: 44-52
DOI: 10.3923/ajpp.2018.44.52
In vitro Culture Development and Secondary Metabolites of Dillenia indica Tree
Lobna S. Taha , Eman A. Ibrahim, Nora M. Youssef and Iman M. El-Sayed

Abstract:
Background and Objective: There are some factors that affected the in vitro behavior of Dillenia indica and secondary metabolites such as different types of culture media, concentrations of BA and NAA as well as the physical state. The aim of this experiment was to study various factors on behaviors of Dillenia indica to attain suitable micropropagation protocol in vitro and in vivo stages. Materials and Methods: The experimental study was carried on Dillenia indica plant to evaluate the effect of three types of different media (MS, WPM and B5) with BA (0.5, 1.0 and 2.0 mg L–1), three physical states (Solid, semi-Solid and liquid) of MS medium with NAA (0.0, 0.2, 0.4 and 0.6 mg L–1) on various morphological and secondary metabolites characters. Results: The maximum numbers of the formed shootlets/explants and leaves/shootlet were recorded for MS medium or WPM+2 mg L–1 of BA while, B5 medium led to reduce those characters. Low concentration of BA (0.5 mg L–1) supplemented to WPM or B5 medium had stimulation effect on shootlets elongation. The maximum phenol, tannin and pigments contents were found in shootlets that were cultured on WPM+1.0 mg L–1 BA. Using various culture media in vitro showed the highest antioxidant capacity as compared to control. Semi solid MS medium+NAA at 0.6 mg L–1 produced the maximum number of roots for the two culture periods and the longest roots in the first period. Culturing the shootlets on liquid MS+NAA at 0.4 mg L–1 resulted in strong and thick roots. The plants were highly survived and attained to highest height when the in vitro rooted plantlets were acclimatized to soil mixture of peat moss alone or peat moss+perlite+sand. Also, the maximum phenols content as well as antioxidant activity were detected in leaves of acclimatized plant in soil mixture of peat moss+sand+clay. Conclusion: The MS medium or WPM with 2 mg L–1 of BA was favored for in vitro propagation. Semi solid MS medium plus NAA at 0.6 mg L–1 produced the maximum number of roots. The maximum phenol, tannin and pigments contents were found in shootlets that were cultured in vitro on WPM medium plus 1.0 mg L–1 BA. The plants were successfully acclimatized in soil mixture of peat moss alone or peat moss+perlite+sand.
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How to cite this article:

Lobna S. Taha, Eman A. Ibrahim, Nora M. Youssef and Iman M. El-Sayed, 2018. In vitro Culture Development and Secondary Metabolites of Dillenia indica Tree. American Journal of Plant Physiology, 13: 44-52.

DOI: 10.3923/ajpp.2018.44.52

URL: https://scialert.net/abstract/?doi=ajpp.2018.44.52

 
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