This study seek to establish the medicinal relevance of the fruit mesocarp of Voacanga africana, using standard methods of microbial sensitivity test and phytochemical analysis. Dried mesocarp samples of V. africana were extracted with hot and cold absolute ethanol. The extracts were screened for their phytochemical composition and antimicrobial activities. The results revealed the presence of some bioactive compounds; alkaloids, anthranoids, anthraquinone, glycosides, saponins, starch and tannins. The crude extracts exhibited antimicrobial activity against Escherichia coli (30.7%), Serratia marcescens (37.5%) and Staphylococcus aureus (39.47%). Others are Alternaria solani (30%), Candida albicans (26%) and Rhizopus stolonifer (20%); relative to the standard antibiotics, Gentamicin and Clotrimazole. The Agar Well Diffusion, sensitivity test, also showed zones of inhibition of 8 and 7.5 mm for hot and cold ethanol extracts, against E. coli, 9 and 00 mm; against S. marcescens, 7.5 and 6.5 mm; against S. aureus, 9 and 8 mm; against A. solani, 7 and 7 mm; against C. albicans, 6.5 and 00 mm, against R. stolonifer, at 100 g mL-1 concentration of the extracts. The hot and cold extracts were equipotent, against C. albicans but vary against other test organisms.