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American Journal of Plant Physiology
  Year: 2007 | Volume: 2 | Issue: 6 | Page No.: 373-377
DOI: 10.3923/ajpp.2007.373.377
 
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Influence of Growth Retardants on Serpentine Accumulation in Catharanthus roseus Cell Suspension Cultures

Magdi El-Sayed and Rob Verpoorte

Abstract:
The effect of three growth retardants Chlormequat chloride, AMO 1618 and Tetcyclacis on accumulation of serpentine in Catharanthus roseus cell culture was studied. These products are known to inhibit gibberellin biosynthesis in the cell. Low and high concentrations of growth retardants were applied to C. roseus cultures and all of them affect growth and accumulation of serpentine. All of these substances lowered cell mass growth at high concentrations compared with control. All of tested compounds increased the content of serpentine compared to the control. High concentration of AMO-1618 resulted in two-fold increase in serpentine accumulation (25.94±1.5 μg g-1 FW) at day 8 after treatment in the cells and the secretion of serpentine to the medium was decreased with time. Chlormequat chloride at high concentration resulted in serpentine productivity of 18.6±0.8 μg g-1 FW at day 1, but no effect on secretion in the medium. Tetcyclacis resulted in lysis of the cells and consequently release of serpentine to the medium.
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How to cite this article:

Magdi El-Sayed and Rob Verpoorte, 2007. Influence of Growth Retardants on Serpentine Accumulation in Catharanthus roseus Cell Suspension Cultures. American Journal of Plant Physiology, 2: 373-377.

DOI: 10.3923/ajpp.2007.373.377

URL: https://scialert.net/abstract/?doi=ajpp.2007.373.377

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