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American Journal of Plant Physiology
  Year: 2007 | Volume: 2 | Issue: 3 | Page No.: 206-213
DOI: 10.3923/ajpp.2007.206.213
 
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Exploration of Ochratoxin A Contamination and its Management in Rice

K.R.N. Reddy, C.S. Reddy and K. Muralidharan

Abstract:
Aspergillus ochraceus contamination was detected in the forty six rice samples collected from areas exposed to rain or flood or stored in godown or mills. These isolates were grown on Pusa basmati rice grains for 7 days at room temperature than extracted the Ochratoxin A (OTA) and identified on TLC plate along with standard in different solvent systems. The OTA was confirmed by changing the colour from greenish blue to blue after spraying 6% NaOH in 20% ethanol on TLC plate. Garlic bulb extract (10%) proved significantly more effective with complete inhibition of A. ochraceus. Pongamia glaberima kernel extract (20%) showed 71% inhibition on mycelial growth of A. ochraceus. Among the culture filtrate of the biocontrol agent, Trichoderma virens inhibited completely while the culture filtrate from T. harzianum showed a partial inhibition (67%) at 15% concentration. The culture filtrate of Pseudomonas fluorescens (DRPf 002) were effectively inhibited (98%) mycelial growth at 15% concentration. Among the fungicides tested carbendazim was effective even at 100 ppm in inhibiting A. ochraceus. Propineb completely reduced the growth at 750 ppm. Among the chemicals tested benzoic acid were completely inhibited the mycelial growth at 0.1% concentration.
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How to cite this article:

K.R.N. Reddy, C.S. Reddy and K. Muralidharan, 2007. Exploration of Ochratoxin A Contamination and its Management in Rice . American Journal of Plant Physiology, 2: 206-213.

DOI: 10.3923/ajpp.2007.206.213

URL: https://scialert.net/abstract/?doi=ajpp.2007.206.213

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