Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
American Journal of Food Technology
  Year: 2011 | Volume: 6 | Issue: 11 | Page No.: 951-962
DOI: 10.3923/ajft.2011.951.962
Validation of the Micro Biological Survey Method for Total Viable Count and E. coli in Food Samples
Giorgia Bottini, Francesca Losito, Alessio De Ascenti, Francesca Romana Priolisi, Alberto Mari and Giovanni Antonini

Abstract:
The aim of this study was the validation of the Micro Biological Survey (MBS) method for microbiological analysis of food for Total Viable Count (TVC) and Escherichia coli (E. coli). The MBS method is a rapid quantitative alternative method for the detection and selective counting of bacteria in agro-food, in water and in environmental samples. It is based on colorimetric survey in mono-use disposable reaction vials that must be filled with the samples without any preliminary treatment (e.g., homogenization, dilution, etc.); the greater the number of bacteria presents into the sample, the faster the color change. However, an independent evaluation of the analytical results obtained with MBS method would be required before commercialization. Therefore, this alternative method was validated in comparison to the reference method. The general estimate of precision, reliability, uncertainty, linearity, accuracy and selectivity were determined. All the performance parameters have demonstrated total correlation between the alternative method and the reference method for the detection and counting of TVC and E. coli both in artificially contaminated and in naturally contaminated samples. MBS assay can be used as rapid and user friendly screening method for detection of TVC and E. coli in food industry.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Comparison of Uncertainty Between Traditional and Alternative Methods for Food Microbiological Analysis
  •    Qualitative and Quantitative Validation of the Micro Biological Survey Method for Listeria spp., Salmonella spp., Enterobacteriaceae and Staphylococcus aureus in Food Samples
  •    Detection of Diarrheagenic Escherichia coli Isolated Using Molecular Approaches
  •    Evaluation of Commercial Colilert18-Quantitray® Method by ISO Techniques for Enumeration and Quantification of Total Coliforms and Escherichia coli in Drinking-Water of Buraidah, Saudi Arabia
  •    Efficacy of the FreshLight® 210 Ultraviolet Light System1 for Control and Elimination of Escherichia coli O157:H7 in a Commercial Beef Brine Solution (Flow Rate = 10 GPM and Turnover Time = 2.5 min)
  •    Monitoring Enterohaemorrhagic Escherichia coli O157:H7 in the Vegetable Food Chain in Ghana
How to cite this article:

Giorgia Bottini, Francesca Losito, Alessio De Ascenti, Francesca Romana Priolisi, Alberto Mari and Giovanni Antonini, 2011. Validation of the Micro Biological Survey Method for Total Viable Count and E. coli in Food Samples. American Journal of Food Technology, 6: 951-962.

DOI: 10.3923/ajft.2011.951.962

URL: https://scialert.net/abstract/?doi=ajft.2011.951.962

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 

       

       

Curve Bottom