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American Journal of Food Technology
  Year: 2011 | Volume: 6 | Issue: 4 | Page No.: 336-341
DOI: 10.3923/ajft.2011.336.341
 
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Study of Antibacterial Activity of Ocimum sanctum Extract Against Gram Positive and Gram Negative Bacteria

Poonam Mishra and Sanjay Mishra

Abstract:
This study was carried out to observe the antibacterial activity of aqueous extract, chloroform extract, alcohol extract and oil obtained from leaves of Ocimum sanctum against the selected bacteria i.e., E. coli, P. aeruginosa, S. typhimurium and S. aureus. The antibacterial activity of Ocimum was evaluated by liquid inhibition test. E. coli, P. aeruginosa, Staphylococcus aureus and Salmonella typhimurium were not found resistant against the Ocimum extract since reduction in optical density were observed from 0.20 to 0.85. Chloroform extract were found most effective against P. aeruginosa where 0.85 reductions in O.D were observed. Extract obtained from Ocimum sanctum were observed equally effective against the gram negative and gram positive bacteria. Present investigation reveals that Ocimum sanctum may be a better alternative as a preservative in Food Industries since it is equally effective against pathogenic gram positive and gram negative bacteria.
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How to cite this article:

Poonam Mishra and Sanjay Mishra, 2011. Study of Antibacterial Activity of Ocimum sanctum Extract Against Gram Positive and Gram Negative Bacteria. American Journal of Food Technology, 6: 336-341.

DOI: 10.3923/ajft.2011.336.341

URL: https://scialert.net/abstract/?doi=ajft.2011.336.341

COMMENTS
13 November, 2010
sheikh sayir aftab:
i firstly thank to the authers coz by reading this i got a lot of knowledge regarding ocimum sanctum .
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