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Asian Journal of Epidemiology
  Year: 2017 | Volume: 10 | Issue: 3 | Page No.: 123-127
DOI: 10.3923/aje.2017.123.127
Molecular Characterization of Orientia tsutsugamushi in Domestic Rodents In Kolar Region, Karnataka
Gangannagari Vijay Kumar and Parvangada Madappa Beena

Abstract:
Background and Objective: Orientia tsutsugamushi (O. tsutsugamushi) is the causative agent of scrub typhus, which is an acute febrile illness seen in human beings. Rodents were implicated as the natural reservoirs for Orientia tsutsugamushi and they served as hosts for chigger mite. This study was undertaken to investigate the molecular prevalence and characterization of Orientia tsutsugamushi in domestic rodents in Kolar region. Methodology: Blood samples were collected from 177 rodents (138 Bandicota bengalensis, 16 Rattus rattus, 9 Bandicota indica, Rattus norvegicus and 2 Mus musculus). Conventional PCR, targeting the 56 kDa type specific gene was performed and DNA sequenced. Results: O. tsutsugamushi DNA was detected by conventional PCR in 10 (5.6 %) rodents. Two PCR purified samples were sequenced, phylogenetic analyses showed that one strain was closely related to Orientia chuto Dubai strain and another was closely related to Boryang strain. Conclusion: This suggests a high incidence of scrub typhus in this region. Further research on the characterization of O. tsutsugamushi from rodents as well as vectors throughout this district is needed to study the prevalence of O. tsutsugamushi in reservoir hosts.
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How to cite this article:

Gangannagari Vijay Kumar and Parvangada Madappa Beena, 2017. Molecular Characterization of Orientia tsutsugamushi in Domestic Rodents In Kolar Region, Karnataka. Asian Journal of Epidemiology, 10: 123-127.

DOI: 10.3923/aje.2017.123.127

URL: https://scialert.net/abstract/?doi=aje.2017.123.127

 
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