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Asian Journal of Crop Science
  Year: 2014 | Volume: 6 | Issue: 2 | Page No.: 133-141
DOI: 10.3923/ajcs.2014.133.141
Improved Androgenesis of Broccoli (Brassica oleracea var italica) Anthers Using Sucrose and Growth Regulators
Magdi A.A. Mousa, Ashraf G. Haridy, Hassan S. Abbas and Mohammed F. Mohammed

Abstract:
The presented study was conducted to study the effects of BAP and its combinations with 2, 4-D and sucrose concentrations on androgenesis of broccoli cv. ‘Arcadia’. The MS Basel media supplemented with AC (0.5 g L-1) and 3% sucrose was used. The experiment was laid out in Completely Randomized Design (CRD) using 3 replicates. The effects of BAP in combination with 2, 4-D and sucrose concentrations on androgenesis of broccoli (cv Arcadia) anther cultures were investigated. The MS basal media supplemented with activated charcoal (AC) (0.5 g L-1) was used. The medium complemented with BAP (1 mg L-1) plus 2, 4-D (0.5 mg L-1) significantly increased the percentage of anthers developing embryos (90%). Increasing 2, 4-D concentration (1 mg L-1) significantly decreased the percentage of anthers formed embryos (10%). Regarding the regeneration of the formed embryos, the culture medium containing BAP (1 mg L-1)+2, 4-D (0.5 mg L-1) or BAP (1 mg L-1)+2, 4-D (1 mg L-1) produced the high percentages of embryos induced callus. The maximum percentage of embryos developed into plantlets were obtained with culture medium with BAP (1 mg L-1)+2, 4-D (0.5 mg L-1) followed by BAP (1 mg L-1) and 2, 4-D (1 mg L-1). Concerning sucrose concentration, the culture medium with low sucrose concentration (20 g L-1) enhanced the percentage of anthers developed embryos. The higher sucrose concentrations (50 and 60 g L-1) increased percentages of dead anthers and anthers formed callus and decreased the percentage of embryos developing plantlets. BAP in combinations with 2, 4-D and low concentrations of sucrose can be successfully used to perform embryos and/or callus from the callus induction and embryos development either to callus or plants.
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How to cite this article:

Magdi A.A. Mousa, Ashraf G. Haridy, Hassan S. Abbas and Mohammed F. Mohammed, 2014. Improved Androgenesis of Broccoli (Brassica oleracea var italica) Anthers Using Sucrose and Growth Regulators. Asian Journal of Crop Science, 6: 133-141.

DOI: 10.3923/ajcs.2014.133.141

URL: https://scialert.net/abstract/?doi=ajcs.2014.133.141

 
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