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Asian Journal of Cell Biology
  Year: 2019 | Volume: 14 | Issue: 1 | Page No.: 1-6
DOI: 10.3923/ajcb.2019.1.6
 
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Monopolar Spindle Induced by Isoamericanol A Suppresses Human Breast Cancer Cell (MCF-7) Growth
Ayako Katagi , Li Sui, Kazuyo Kamitori, Toshisada Suzuki, Takeshi Katayama, Youyi Dong, Toshitaka Nakagawa, Koichi Yube, Akram Hossain, Fuminori Yamaguchi and Masaaki Tokuda

Abstract:
Background and Objective: The neolignan isoamericanol A was purified from the organic layer of the MeOH extract of the waste residue of the Jatropha curcas L. (Jatropha) seed. The previous work revealed that isoamericanol A can induce G2/M arrest on MCF-7. The aim of the present study was to evaluate the selective effect of isoamericanol A on the human breast cancer cell line (MCF-7), as well as to clarify the point at which isoamericanol A exhibited anti-cancer activity on MCF-7 at M phase. Materials and Methods: The normal human breast cell line (MCF-10A) and the human breast cancer cell line (MCF-7) were subjected to isoamericanol A treatment for 3 days for cell growth inhibition assay. DNA microarray analysis was performed to reveal the expressional difference on MCF-7 with or without 3-day isoamericanol A treatment. Also, the effect of short term isoamericanol A treatment was studied by flow cytometry analysis, as well as immunofluorescent staining using Aurora B, α-tubulin and the chromosome. Results: In this experiment, both MCF-10A and MCF-7 were treated with isoamericanol A to measure the relative degrees of inhibition. Though inhibiting both cell lines, isoamericanol A effected 25.9±6.14% (n = 4, p<0.05) more growth inhibition on MCF-7 than it did on MCF-10A. Microarray analysis showed isoamericanol A resulted in numerous expressional changes in cell division related genes pertaining to spindle formation. The immunofluorescent staining showed that brief isoamericanol A treatment is capable of inducing cell growth inhibition specifically by disrupting regular spindle formation during M phase. Flow cytometry analysis showed brief isoamericanol A treatment induced G2/M arrest. Conclusion: Isoamericanol A inhibits human breast cancer cell proliferation more than it does normal human breast cells. Moreover, this study revealed that isoamericanol A treatment can induce G2/M cell cycle arrest due to monopolar spindle formation during cell division.
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How to cite this article:

Ayako Katagi, Li Sui, Kazuyo Kamitori, Toshisada Suzuki, Takeshi Katayama, Youyi Dong, Toshitaka Nakagawa, Koichi Yube, Akram Hossain, Fuminori Yamaguchi and Masaaki Tokuda, 2019. Monopolar Spindle Induced by Isoamericanol A Suppresses Human Breast Cancer Cell (MCF-7) Growth. Asian Journal of Cell Biology, 14: 1-6.

DOI: 10.3923/ajcb.2019.1.6

URL: https://scialert.net/abstract/?doi=ajcb.2019.1.6

 
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