Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Asian Journal of Cell Biology
  Year: 2007 | Volume: 2 | Issue: 2 | Page No.: 50-53
DOI: 10.3923/ajcb.2007.50.53
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Luteinizing Hormone (LH) Effects on In vitro Nuclear Maturation of Bovine Oocytes
M. Al-Amin, A. Nahar and M.S. Ali

Abstract:
The present study was undertaken to investigate the effects of Luteinizing Hormone (LH, 100 ng mL-1) on bovine oocyte maturation in vitro (Experiment 1) and the relationship between the gap junction and LH (Experiment-2). In experiment 1, cumulus-enclosed oocyte (CO) and Denuded Oocytes (DO) were cultured in the presence or absence of LH. After 42-43 h in vitro culture, nuclear maturation rates which reached the metaphase stage of the second meiotic division (M-ll stage) were observed. In Experiment 2, we evaluated whether the gap junction, formed between cumulus cells and oocytes, was associated with the presence of LH in the maturation medium. The addition of LH to the maturation medium significantly enhanced nuclear maturation of both CO and DO (p<0.05) compared with those oocytes, which were cultured without LH. The addition of 1-heptanol (5 mM), a blocker of coupling in the gap junction, to the maturation medium supplemented with LH significantly inhibited (p<0.05) the number of CO and DO reaching the M-ll stage. These results suggest that the addition of LH to the maturation medium could improve the nuclear maturation of bovine oocytes.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Relations Between GDF9, Zar1, Mater and DNMT1 Gene Relative mRNA Abundance and Oocytes Quality of Ovine Oocytes
How to cite this article:

M. Al-Amin, A. Nahar and M.S. Ali, 2007. Luteinizing Hormone (LH) Effects on In vitro Nuclear Maturation of Bovine Oocytes . Asian Journal of Cell Biology, 2: 50-53.

DOI: 10.3923/ajcb.2007.50.53

URL: https://scialert.net/abstract/?doi=ajcb.2007.50.53

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 

       

       

Curve Bottom