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Asian Journal of Biological Sciences
  Year: 2019 | Volume: 12 | Issue: 4 | Page No.: 772-778
DOI: 10.3923/ajbs.2019.772.778
Anti-sickling Properties of Aqueous Extracts of Dennettia tripetala and Physalis angulata
B.M. Onyegeme-Okerenta , E.B. Essien and J.I. Esin

Abstract:
Background and Objective: Sickle cell disease (SCD) is a group of genetically inherited blood disorders typically passed from the parent to the offspring and is characterized by an abnormality and a reduction in the oxygen carrying capacity of the haemoglobin molecule of erythrocytes. This study was designed to investigate the anti-sickling properties of aqueous leaf extracts of Physalis angulata and Dennettia tripetala on homozygous sickle cell erythrocyte. Materials and Method: The experiment was in four groups; Group 1 (Normal RBC), Group 2 (Sickle RBC-experimental control group), Group 3 (Sickle RBC+D. tripetala) and Group 4 (Sickle RBC+P. angulata). Fresh blood was collected from a sickle cell patient and normal individual which were used for the study. Osmotic fragility, total anti-oxidants (Glutathione, Superoxide dismutase and Catalase activity test), anti-sickling and gelatin test were carried out on the samples in vitro. Results: Result of total anti-oxidants activities showed that there was a significant decrease (p<0.05) in GSH (3.63±0.015) and SOD (0.35±0.015) and a significant increase (p<0.05) in catalase (7.34±0.021) activities when the experimental control group (Group 2) was compared with Group 1. However, treatment of sickled RBCs with extracts of D. tripetala and P. angulata induced a significant increase (p<0.05) in GSH (3.63±0.015 and 3.92±0.008) and SOD 0.35±0.015 and 0.37±0.005) and a significant decrease (p<0.05) in catalase activities (7.34±0.021 and 8.11±0.004) respectively, when compared with the experimental control group (Group 2). Antisickling effect also showed that, 1 mL of 100 mg mL1 concentration of D. tripetala and P. angulata with the sickle RBC, in the ratio of 1:1, reduced the abnormal cell to 13, 9 and 6% with D. tripetala and 11, 8 and 6% with P. angulata at a time interval of 20, 40 and 60 min, respectively. Osmotic fragility showed an increase in membrane-stability when P. angulata and D. tripetala leaf extract were added, while gelatin test showed a reduction in haemoglobin polymerization at a 0.8 mg/100 mL concentration of the extracts. Conclusion: From the findings of this study, it can be concluded that D. tripetala leaf extract and P. angulata have antisickling potentials and may be used for therapeutic management of sickle cell anaemia.
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How to cite this article:

B.M. Onyegeme-Okerenta, E.B. Essien and J.I. Esin, 2019. Anti-sickling Properties of Aqueous Extracts of Dennettia tripetala and Physalis angulata. Asian Journal of Biological Sciences, 12: 772-778.

DOI: 10.3923/ajbs.2019.772.778

URL: https://scialert.net/abstract/?doi=ajbs.2019.772.778

 
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