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Asian Journal of Biological Sciences
  Year: 2019 | Volume: 12 | Issue: 3 | Page No.: 610-615
DOI: 10.3923/ajbs.2019.610.615
 
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Antimicrobial Activities of in vitro and in vivo Extract Sources of Acacia senegal
Hussien M. Daffalla , Kauther Sir Elkhatim Ali, Huda Abdirizak Jama Hassan, Azza M. Elsheikh and Magdoleen G. Osman

Abstract:
Background and Objective: Various research studies reported high contents of bioactive compounds in extracts of Acacia senegal. Therefore, this study aims to evaluate the antimicrobial activity of A. senegal as affected by the extract source. Materials and Methods: Three different type of extract viz. callus, in vitro shoot and in vivo leaf parts were examined. For callus induction, hypocotyl and cotyledon explants were cultured on Murashige and Skoog (MS) medium fortified with 2, 4-dichlorophenoxyacetic acid (2, 4-D) and naphthalene acetic acid (NAA) (0-3 mg mL1) alone or in combination with kinetin (Kin) or 6-benzyladenine (BA) (0.5-3 mg mL1). The three parts of A. senegal were macerated in methanol then extracts tested against four pathogenic microbes using agar well diffusion method. Results: The best callus induction frequency was attained in hypocotyl explant cultured on MS medium fortified with 3 mg L1 of 2, 4- D and NAA. Callusing was further improved as 2, 4- D and NAA combined with BA and kin lead to high production obtained with 3 mg mL1 2, 4- D and 1 mg mL1 kin. The results on anti-microbial activity showed substantial growth inhibition by in vitro shoot extract followed with in vivo leaf extract and then callus extract. The significantly maximum inhibition zones 23 mm were achieved by in vitro shoot extract at 50 mg mL1 against Escherichia coli. Conclusion: Callus induction was accomplished on hypocotyl explant of A. senegal. The preliminary antimicrobial screening of A. senegal extracts showed variable activities depending on the extract source.
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How to cite this article:

Hussien M. Daffalla, Kauther Sir Elkhatim Ali, Huda Abdirizak Jama Hassan, Azza M. Elsheikh and Magdoleen G. Osman, 2019. Antimicrobial Activities of in vitro and in vivo Extract Sources of Acacia senegal. Asian Journal of Biological Sciences, 12: 610-615.

DOI: 10.3923/ajbs.2019.610.615

URL: https://scialert.net/abstract/?doi=ajbs.2019.610.615

 
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