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Asian Journal of Biological Sciences
  Year: 2019 | Volume: 12 | Issue: 2 | Page No.: 156-163
DOI: 10.3923/ajbs.2019.156.163
 
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Frequency and Spectrum of Chlorophyll Mutations Induced by Single and Combination Treatments of Gamma Rays and EMS in Urdbean

Sonu Goyal, Mohammad Rafiq Wani and Samiullah Khan

Abstract:
Background and Objective: Chlorophyll mutation frequency is useful in assessing the potency of a mutagen. Hence, scoring of chlorophyll mutations are dependable indices for evaluating the genetic effects of mutagenic treatments. Present investigation was undertaken to understand the response of urdbean varieties T-9 and Pant U-30 to individual and combined treatments of gamma rays and EMS for identifying such mutagenic treatments causing maximum chlorophyll mutations which could be exploited for inducing beneficial viable mutations in subsequent generations. Materials and Methods: Seeds of two urdbean varieties were irradiated with 100, 200, 300 and 400 Gy doses of gamma rays. For chemical treatments, seeds were treated with 0.1-0.4% of EMS for 6 h and for combination treatments, dry seeds of each variety were first irradiated with gamma rays at 200 and 300 Gy followed by the treatment with 0.2 and 0.3% of EMS. The mutagen treated seeds were sown in complete randomized block design to raise M1 generation. The M1 seeds were sown in next season in plant progeny rows for raising M2 generation. Chlorophyll mutations were observed when seedlings were 8-15 days old in M2 generation. They were identified and classified according to Gustafson’s method. Results: Six kinds of chlorophyll mutants viz., albina, chlorina, maculata, xantha, virescent and viridis were recorded in M2 generation when seedlings were 7-15 days old. Out of the six chlorophyll mutation types, albina, chlorina and xantha survived upto 7-15 days only, while the other three types were viable and set seeds. Gamma rays alone as well as in combination with EMS produced large number of albina mutations, while EMS alone induced the maximum number of chlorina mutations in both the varieties. Frequency of chlorophyll mutations was dose dependent and increased with increasing dose of each mutagen. The combined treatments of gamma rays+EMS produced higher frequency of chlorophyll mutations as compared to their individual treatments. Conclusion: Chlorophyll mutations could be exploited for identifying the threshold dose of a mutagen that would increase the probability of retrieving economically useful mutations in ensuing generations.
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How to cite this article:

Sonu Goyal, Mohammad Rafiq Wani and Samiullah Khan, 2019. Frequency and Spectrum of Chlorophyll Mutations Induced by Single and Combination Treatments of Gamma Rays and EMS in Urdbean. Asian Journal of Biological Sciences, 12: 156-163.

DOI: 10.3923/ajbs.2019.156.163

URL: https://scialert.net/abstract/?doi=ajbs.2019.156.163

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