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Asian Journal of Biological Sciences

Year: 2017 | Volume: 10 | Issue: 2 | Page No.: 64-71
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Authors

Md. Fakruddin

Md. Fakruddin

Md. Mizanur Rahaman

Md. Nur Hossain

Monzur Morshed Ahmed

Keywords

Research Article

Induction and Resuscitation of Cronobacter sakazakii into Viable but Non-culturable State at Low Temperature in Water Microcosm

Md. Fakruddin Md. Fakruddin's LiveDNA, Md. Mizanur Rahaman, Md. Nur Hossain and Monzur Morshed Ahmed
Background and Objective: Cronobacter sakazakii considered to be an important human pathogen and has been increasingly linked to food-borne illness. This study aimed to investigate the fate of C. sakazakii in lake water under low temperature and to evaluate the potential hazard of viable but nonculturable (VBNC) cells induced under such stressful conditions. Methodology: A laboratory strain of Cronobacter sakazakii was induced into VBNC state at low temperature in water microcosm and resuscitation and virulence gene was examined. Effect of growth phase and inoculum level on induction into VBNC was determined. Results were analysed by student’s t-test. Results: Results suggested that C. sakazakii strain can enter into VBNC state at low temperature. Growth phase and inoculum level has no effect on induction of VBNC. The VBNC forms of the strain examined can be resuscitated by temperature upshift and addition of catalase. The VBNC C. sakazakii strain showed the potential to retain virulence. Virulence gene expression and alteration of protein secretion was observed during VBNC state. Conclusion: It is concluded that under some stress conditions, Cronobacter sakazakii can not only enter into VBNC state but also retain virulence and resuscitation potential. This study will help researchers to explore mechanisms of survival and virulence of the pathogen in food value chain. Understanding these mechanisms may pave the way to device new control strategies.
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How to cite this article

Md. Fakruddin, Md. Mizanur Rahaman, Md. Nur Hossain and Monzur Morshed Ahmed, 2017. Induction and Resuscitation of Cronobacter sakazakii into Viable but Non-culturable State at Low Temperature in Water Microcosm. Asian Journal of Biological Sciences, 10: 64-71.

URL: https://scialert.net/abstract/?doi=ajbs.2017.64.71

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