The pathogenesis of vitiligo is still not clear. The relationship between human leukocyte antigen (HLA) and vitiligo is controversial. The aim of this study was to evaluate the HLA class I in patients suffering from vitiligo. In this analytical case-control study, venous blood samples of 50 healthy subjects as control and 50 patients with vitiligo are evaluated to determine the type of human leukocyte antigens with microcytotoxicity technique. The control group was selected from healthy kidney donors that were typed for HLA class I antigens. HLA-A2 (P = 0.009, OR = 2.901), B49 (P = 0.031, OR = 9.333), CW2 (P = 0.008, OR = 10.756), CW3 (P = 0.031, OR = 9.333), CW7(P = 0.008, OR = 10.756) were determined as predisposing antigens and HLA-A3 (P = 0.019, OR = 0.316), B8 (P = 0.031, OR = 0.107), BW4 (P = 0.000, OR = 0.008), BW6 (P = 0.001, OR = 0.206) as preventive antigens for the disease. HLA A2, HLA B49, HLA CW2, HLA CW3 and HLA CW7 are regarded as genetic susceptible factors for manifestation of the disease and HLA A3, HLA B8, HLA BW4 and HLA BW6 as genetic susceptible factors for manifestation of vitiligo.