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Asian Journal of Biological Sciences
  Year: 2013 | Volume: 6 | Issue: 5 | Page No.: 257-264
DOI: 10.17311/ajbs.2013.257.264
 
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Biological and Molecular Characterization of Potato virus Y Infecting Potato (Solanum tuberosum) in India

Pooja Sharma, Anurag Kumar Sahu, Rakesh Kumar Verma, Ritesh Mishra and R.K. Gaur

Abstract:
A survey conducted in potato growing tracts of Rajasthan (India), covering 25 fields in 15 villages, revealed the occurrence of potato mosaic disease. A potyvirus (Potato virus Y, PVY) causing severe mosaic mottling in potato (Solanum tuberosum) was identified. The aim of this study to characterize this potyvirus on the basis of biological, serological and partial nucleotide sequence properties from different locations of Rajasthan. Electron microscopy of this virus revealed flexuous filamentous virus particles of 750x15 nm and showed positive result against antiserum specific for PVY. RT-PCR assay was assessed using potyvirus specific primers designed against the core region of the Nib and Coat Protein (CP). cDNA fragments of Nib and CP gene, 323 and 353 bp were obtained, respectively. Sequencing and analysis of the amplicons showed that the virus is closely related to Potato virus Y. Sequence analysis reveals that virus isolate (PVY-PS) showed 93-97% similarity with other worldwide PVY isolates. Nucleotide sequences have been submitted to NCBI data base under the accession number-KC753451and KC753450.
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How to cite this article:

Pooja Sharma, Anurag Kumar Sahu, Rakesh Kumar Verma, Ritesh Mishra and R.K. Gaur, 2013. Biological and Molecular Characterization of Potato virus Y Infecting Potato (Solanum tuberosum) in India. Asian Journal of Biological Sciences, 6: 257-264.

DOI: 10.17311/ajbs.2013.257.264

URL: https://scialert.net/abstract/?doi=ajbs.2013.257.264

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